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291.

Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin

Nam-Gun Kim, Myeong-Ae Kim, Young-Il Park, Tae-Sung Jung, Seong-Wan Son, ByungJae So, Hwan-Goo Kang 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 6 431-437 (7 pages)

Nam-Gun Kim, Myeong-Ae Kim, Young-Il Park, Tae-Sung Jung, Seong-Wan Son, ByungJae So, Hwan-Goo Kang 저
...
- 3,000원
Monoclonal anti-enrofloxacin antibody was prepared for a direct competitive enzyme-linked immunosorbent assay (ELISA) and purification system using monoclonal antibody (mAb) coupled magnetic nanoparticles (MNPs). The IC50 values of the developed mAb for enrofloxacin (ENR), ciprofloxacin, difloxacin, sarafloxacin, pefloxacin, and norfloxacin were 5.0, 8.3, 9.7, 21.7, 36.0, and 63.7 ng/mL, respectively. The lowest detectable level of ENR was 0.7 ng/mL in the prepared ELISA system. To validate the...
292.

Elevated level of renal xanthine oxidase mRNA transcription after nephropathogenic infectious bronchitis virus infection in growing layers

Huayuan Lin, Qiqi Huang, Xiaoquan Guo, Ping Liu, Weilian Liu, Yuelong Zou, Shuliang Zhu, Guangfu Deng, Jun Kuang, Caiying Zhang, 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 5 423-429 (7 pages)

Huayuan Lin, Qiqi Huang, Xiaoquan Guo, Ping Liu, Weilian Liu, Yuelong Zou, Shuliang Zhu, Guangfu Deng, Jun Kuang, Caiying Zhang, 저
...
- 3,000원
To assess relationships between xanthine oxidase (XOD) and nephropathogenic infectious bronchitis virus (NIBV) infection, 240 growing layers (35 days old) were randomly divided into two groups (infected and control) of 120 chickens each. Each chicken in the control and infected group was intranasally inoculated with 0.2 mL sterile physiological saline and virus, respectively, after which serum antioxidant parameters and renal XOD mRNA expression in growing layers were evaluated at 8, 15 and 22...
293.

Stress and pain response after oligofructose induced-lameness in dairy heifers

Hedie A. Bustamante, Alfredo R. Rodr?guez, Daniel E. Herzberg, Marianne P. Werner 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 3 405-411 (7 pages)

Hedie A. Bustamante, Alfredo R. Rodr?guez, Daniel E. Herzberg, Marianne P. Werner 저
...
- 3,000원
Lameness is one of the most painful conditions that affects dairy cattle. This study was conducted to evaluate clinical signs and plasma concentration of several pain and stress biomarkers after oligofructose-induced lameness in dairy heifers. Lameness was induced using an oligofructose overload model in 12 non-pregnant heifers. Clinical parameters and blood samples were obtained at 48 and 24 h and at 6, 12, 24, 36 and 48 h after induction of lameness. Clinical parameters included heart rate,...
294.

Anti-adipogenic effect of Artemisia annua in diet-induced-obesity mice model

Hye Kyung Baek, Hyeji Shim, Hyunmook Lim, Minju Shim, Chul-Kyu Kim, Sang-Kyu Park, Yong Seok Lee, Ki-Duk Song, Sung-Jo Kim, Sun 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 1 389-396 (8 pages)

Hye Kyung Baek, Hyeji Shim, Hyunmook Lim, Minju Shim, Chul-Kyu Kim, Sang-Kyu Park, Yong Seok Lee, Ki-Duk Song, Sung-Jo Kim, Sun 저
...
- 3,000원
Obesity has increased continuously in western countries during the last several decades and recently become a problem in developing countries. Currently, anti-obesity drugs originating from natural products are being investigated for their potential to overcome adverse effects associated with chemical drugs. Artemisinic acid, which was isolated from the well-known anti-malaria herb Artemisia annua (AA) L., was recently shown to possess anti-adipogenic effects in vitro. However, the...
295.

Rapamycin treatment during in vitro maturation of oocytes improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs

Joohyeong Lee, Jong-Im Park, Jung Im Yun, Yongjin Lee, Hwanyul Yong, Seung Tae Lee, Choon-Keun Park, Sang-Hwan Hyun, Geun-Shik L 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 16 373-380 (8 pages)

Joohyeong Lee, Jong-Im Park, Jung Im Yun, Yongjin Lee, Hwanyul Yong, Seung Tae Lee, Choon-Keun Park, Sang-Hwan Hyun, Geun-Shik L 저
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- 3,000원
This study was conducted to investigate the effects of rapamycin treatment during in vitro maturation (IVM) on oocyte maturation and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Morphologically good (MGCOCs) and poor oocytes (MPCOCs) were untreated or treated with 1 nM rapamycin during 0–22 h, 22–42 h, or 0–42 h of IVM. Rapamycin had no significant effects on nuclear maturation and blastocyst formation...
296.

A multiplex quantitative real-time polymerase chain reaction panel for detecting neurologic pathogens in dogs with meningoencephalitis

Jae-Ik Han, Dong-Woo Chang, Ki-Jeong Na 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 12 341-347 (7 pages)

Jae-Ik Han, Dong-Woo Chang, Ki-Jeong Na 저
...
- 3,000원
Meningoencephalitis (ME) is a common inflammatory disorder of the central nervous system in dogs. Clinically, ME has both infectious and non-infectious causes. In the present study, a multiplex quantitative real-time polymerase chain reaction (mqPCR) panel was optimized for the detection of eight canine neurologic pathogens (Blastomyces dermatitidis, Cryptococcus spp., Neospora caninum, Borrelia burgdorferi, Bartonella spp., Toxoplasma gondii, Ehrlichia canis, and canine distemper virus [CDV])....
297.

Quantitative determination of 12-hydroxyeicosatetraenoic acids by chiral liquid chromatography tandem mass spectrometry in a murine atopic dermatitis model

Seong-Ho Hong, Ji Eun Han, Ji-Seung Ko, Sun Hee Do, Eung Ho Lee, Myung-Haing Cho 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 8 307-315 (9 pages)

Seong-Ho Hong, Ji Eun Han, Ji-Seung Ko, Sun Hee Do, Eung Ho Lee, Myung-Haing Cho 저
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- 3,000원
Atopic dermatitis, one of the most important skin diseases, is characterized by both skin barrier impairment and immunological abnormalities. Although several studies have demonstrated the significant relationship between atopic dermatitis and immunological abnormalities, the role of hydroxyeicosatetraenoic acids (HETE) in atopic dermatitis remains unknown. To develop chiral methods for characterization of 12-HETE enantiomers in a 1-chloro-2,4-dinitrochlorobenzene (DNCB)-induced atopic...
298.

Efficacy of silver ions against Sacbrood virus infection in the Eastern honey bee Apis cerana

Ah-Jin Ahn, Kyu-Sung Ahn, Guk-Hyun Suh, Jin-Hyeong Noh, Young-Ha Kim, Mi-Sun Yoo, Seung-Won Kang, Sung-Shik Shin 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 6 289-295 (7 pages)

Ah-Jin Ahn, Kyu-Sung Ahn, Guk-Hyun Suh, Jin-Hyeong Noh, Young-Ha Kim, Mi-Sun Yoo, Seung-Won Kang, Sung-Shik Shin 저
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- 3,000원
were assigned to the untreated control group in which bees were given syrup without the silver ions. To assess the efficacy of the silver ions, colony strength, colony activity, and the number of dead larvae per hive were measured. During the experimental period, the test group maintained its strength and activity until day 32 while those of bees in the control group decreased sharply after day 8 to 16. Survival duration of the test group was significantly longer (40 days) than that of the...
299.

Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis

Xiao-wei Zhao, Yong-xin Yang, Dong-wei Huang, Guang-long Cheng, Hui-ling Zhao 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 2 253-263 (11 pages)

Xiao-wei Zhao, Yong-xin Yang, Dong-wei Huang, Guang-long Cheng, Hui-ling Zhao 저
...
- 4,000원
Cows infected with Escherichia (E.) coli usually experience severe clinical symptoms, including damage to mammary tissues, reduced milk yield, and altered milk composition. In order to investigate the host response to E. coli  infection and discover novel markers for mastitis treatment, mammary tissue samples were collected from healthy cows and bovines with naturally occurring severe E. coli  mastitis. Changes of mammary tissue proteins were examined using two-dimensional gel...
300.

Quantitative real-time polymerase chain reaction for detecting Mycoplasma hyosynoviae and Mycoplasma hyorhinis in pen-based oral, tonsillar, and nasal fluids 

João Carlos Gomes Neto, Leslie Bower, Barbara Z. Erickson, Chong Wang, Matthew Raymond, Erin L. Strait 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 9 195-201 (7 pages)

João Carlos Gomes Neto, Leslie Bower, Barbara Z. Erickson, Chong Wang, Matthew Raymond, Erin L. Strait 저
...
- 3,000원
Mycoplasma (M.) hyorhinis and M. hyosynoviae are pathogens known to cause disease in pigs post-weaning. Due to their fastidious nature, there is increased need for culture-independent diagnostic platforms to detect these microorganisms. Therefore, this study was performed to develop and optimize quantitative real-time PCR (qPCR) assays to rapidly detect M. hyorhinis and M. hyosynoviae in pen-based oral fluids as well as nasal and tonsillar fluids as proxies for samples used in swine herd...

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