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321.

Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays

In-Soo Choi and Han-Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 10 207-212 (6 pages)

In-Soo Choi and Han-Sang Yoo 저
...
- 3,000원
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2 (rpIL-2) was expressed in Escherichia coli. The size of rpIL-2 without signal peptides was about 15 kDa when determined by SDS-PAGE and Western blotting analysis. Anti-rpIL-2 antibody was produced from mice immunized with the purified rpIL-2, and its specificity was examined by...
322.

Functional Role of Serine Residues of Transmembrane Dopamin VII in Signal Transduction of CB2 Cannabinoid Receptor

Man-Hee Rhee 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 7 185-191 (7 pages)

Man-Hee Rhee 저
...
- 3,000원
Using site-directed mutagenesis technique, I have replaced serine 285 and serine 292 with the alanine, and assessed the binding of agonist and signaling such as the inhibition of adenylyl cyclase activity. I have found that serine 292 has an important role in the signal transduction of cannabinoid agonists, HU-210 and CP55940, but not in that of aminoalkylindoles derivatives WIN55,212-2. All mutants express well in protein level determined by western blot using monoclonal antibody HA 11 as...
323.

Localization of Porcine Reproductive and Respiratory Syndrome Virus Infection in Boars by In Situ Riboprobe Hybridization

Jin-Ho Shin and Thomas W. Molitor 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 5 87-96 (10 pages)

Jin-Ho Shin and Thomas W. Molitor 저
...
- 3,000원
The capability of porcine reproductive and respiratory syndrome virus (PRRSV) to be shed in semen for extended periods of time has been suggested to be a principal factor for viral transmission via insemination. In attempts to gain insights into the mechanism of PRRSV persistence in boars, tissue distribution and sites of viral infection were investigated by in situ hybridization (ISH) using digoxigenin-labeled RNA probe and the ISH results were compared with those of reverse...
324.

Genetic characterization of porcine circovirus - 2 field isolates from PMWS pigs

Jin H. Kim, Young S. Lyoo 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 6 31-39 (9 pages)

Jin H. Kim, Young S. Lyoo 저
...
- 3,000원
of two field isolates (PC201DJ and PC201SS) from pigs showing typical PMWS was sequenced. The nucleotide sequence homology with other PCV-2 isolates was ranging from 95% to 99% in complete viral genomic sequence. The highly conserved nonanucleotide motif of replication origin was identical to that of other PCV-2 isolates. To determine the genetic heterogeneity of PCV-2 isolates, the phylogenetic tree based on the complete genome of PCV-2 isolates were constructed. Two PCV-2 field isolates were...
325.

Characterization of the Recombinant Proteins of Porcine ircovirus Type 2 Field Isolate Expressed in the Baculovirus System

Yu Na Kim, Jin Hyun Kim, Kyoung Soo Kang, Young S. Lyoo 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 4 19-23 (5 pages)

Yu Na Kim, Jin Hyun Kim, Kyoung Soo Kang, Young S. Lyoo 저
...
- 1,500원
Porcine circovirus (PCV) type2 was isolated using primary porcine kidney cells from lymph node of piglets with typical PMWS. The presence of the virus was identified by PCR using primers specific to PCV type2. The ORFs 1 and 2 were amplified by PCR using primers corresponding to the target genes of the PCV type 2. Cloned genes were inserted into the baculovirus expression vector and PCV recombinant proteins were expressed using baculovirus expression system. Recombinant protein expression was...
326.

Cloning a new allele form of bovine TNF-a

Jongsam Ahn 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 12 139-141 (3 pages)

Jongsam Ahn 저
...
- 1,500원
and modulators of immune responses against pathogens and cytokines such as IL-2, IL-7, IL-15 and TNF-a. To study the role TNF-a on gammadelta T lymphocytes, we cloned bovine TNF-a. Sequence analysis revealed that a new allele form of bovine TNF-a was cloned which has 3 additional nucleotide sequences as well as 3 nucleotide substitutions compared with previously reported bovine TNF-a. Further studies are needed to document the functional significance of a new allele form of TNF-a in cattle.
327.

Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos

Gook-jun Ahn, Byeong-chun Lee, Woo-suk Hwang 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 11 131-137 (7 pages)

Gook-jun Ahn, Byeong-chun Lee, Woo-suk Hwang 저
...
- 3,000원
For parthenogenetic activation as a model system of nuclear transfer, microinjection and electroporation as activation treatments in bovine metaphase II oocytes were administered to each of three groups as follows: control group (treatments with Ca2+, Mg2+ -free PBS+100 micro M EGTA), IP3 group (control+25 micro M IP3) and IP3+ ryanodine group (control+25 micro M IP3+10 mM ryanodine). In experiments using microinjection, no significant differences were observed between any of the developmental...
328.

Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6

Il-seob Shin, Hye-ryon Kim, Myung-jin Nam1 and Hwa-young Youn 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 8 115-120 (6 pages)

Il-seob Shin, Hye-ryon Kim, Myung-jin Nam1 and Hwa-young Youn 저
...
- 3,000원
a single open reading frame of 432 nucleotides, which encodes a 143 amino acid polypeptide and has 44.7, 42.4, 37 and 23.7% homology with the cow, sheep, human and rat IL-3 sequences, respectively. Canine IL-6 cDNA (GenBank accession number; AF275796) encodes a putative 20-amino acid signal peptide followed by a 187-amino acid mature protein. The predicted amino acid sequence of canine IL-6 shares 60.4, 77.2, 71.0, 55.8 and 42.0% sequence identity with those of human, feline, porcine, sheep and...
329.

Detection of canine distemper virus (CDV) through one step RT-PCR combined with nested PCR

Yong-Hwan Kim, Kyu-Woan Cho, Hwa-Young Youn, Han Sang Yoo, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 10 59-63 (5 pages)

Yong-Hwan Kim, Kyu-Woan Cho, Hwa-Young Youn, Han Sang Yoo, Hong-Ryul Han 저
...
- 1,500원
A one step reverse transcription PCR (RT-PCR) combined nested PCR was set up to increase efficiency in the diagnosis of canine distemper virus (CDV) infection after developement of nested PCR. Two PCR primer sets were designed based on the sequence of nucleocapsid gene of CDV Onderstepoort strain. One-step RT-PCR with the outer primer pair was revealed to detect 10(2) PFU/ml. The sensitivity was increased hundredfold using the one-step RT-PCR combined with the nested PCR. Specificity of the PCR...
330.

Evaluation of expression patterns of feline CD28 and CTLA-4 in feline immunodeficiency virus (FIV)-infected and FIV antigen-induced PBMC

In-Soo Choi, Han Sang Yoo, Ellen W. Collisson 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 2호 6 97-103 (7 pages)

In-Soo Choi, Han Sang Yoo, Ellen W. Collisson 저
...
- 3,000원
It is known that CD28, a positive costimulatory receptor, plays a very important role in inducing the optimal stimulation of T lymphocytes. CTLA-4 (CD152), however, acts as a negative regulator in T lymphocyte activation. The effect of an feline immunodeficiency virus (FIV) infection on the expression of feline CD28 and CTLA-4 was studied with FIV-infected and uninfected peripheral blood mononuclear cells (PBMC) using a competitive PCR assay. The nature of CD28 and CTLA-4 expression was also...

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