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Paclitaxel inhibits the hyper-activation of spleen cells by lipopolysaccharide and induces cell death
Hyun-Ji Kim, Hong-Gu Joo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 3 453-458 (6 pages)
Paclitaxel was isolated from the bark of the Pacific yew, Taxus brevifolia, and used as an anticancer agent. Paclitaxel prevents cancer cell division by inhibiting spindle fiber function, inducing cell death. A recent study demonstrated that paclitaxel binds to myeloid differentiation protein-2 of Toll-like receptor 4 and prevents the signal transduction of lipopolysaccharide (LPS). Paclitaxel converts immune cells hypo-responsive to LPS. In this study, we investigated whether paclitaxel can... -
Immunologic properties of differentiated and undifferentiated mesenchymal stem cells derived from umbilical cord blood
Hyo-Jong Lee, Kyung-Sun Kang, Sun-Young Kang, Hyung-Sik Kim, Se-Jin Park, Seung-Yong Lee, Kwang-Dong Kim, Hee-Chun Lee, Ji-Kwon 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 4 289-297 (9 pages)
The expression of immunogenic markers after differentiation of umbilical cord blood (UCB)-derived mesenchymal stem cells (MSC) has been poorly investigated and requires extensive in vitro and in vivo testing for clinical application. The expression of human leukocyte antigen (HLA) classes on UCB-derived MSC was tested by Fluorescence-activated cell sorting analysis and immunocytochemical staining. The undifferentiated MSC were moderately positive for HLA-ABC, but almost completely negative for... -
Hydration status affects osteopontin expression in the rat kidney
Su-Youn Lee, Sae-Jin Lee, Hong-Lin Piao, Suk-Young Yang, I. David Weiner, Jin Kim, Ki-Hwan Han 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 2 269-277 (9 pages)
Osteopontin (OPN) is a secretory protein that plays an important role in urinary stone formation. Hydration status is associated with the development of urolithiasis. This study was conducted to examine the effects of dehydration and hydration on OPN expression in the rat kidney. Animals were divided into three groups, control, dehydrated, and hydrated. Kidney tissues were processed for light and electron microscope immunocytochemistry, in situ hybridization, and immunoblot analysis. Dehydration... -
Hematoporphyrin monomethyl ether combined with He?Ne laser irradiation-induced apoptosis in canine breast cancer cells through the mitochondrial pathway
Huatao Li, Jinjin Tong, Jun Bao, Damu Tang, Wenru Tian, Yun Liu 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 13 235-242 (8 pages)
Hematoporphyrin monomethyl ether (HMME) combined with He-Ne laser irradiation is a novel and promising photodynamic therapy (PDT)-induced apoptosis that can be applied in vitro on canine breast cancer cells. However, the exact pathway responsible for HMME-PDT in canine breast cancer cells remains unknown. CHMm cells morphology and apoptosis were analyzed using optical microscope, terminal deoxynucleotidyl transferase dUTP nick end labeling fluorescein staining and DNA ladder assays. Apoptotic... -
Differential regulation of senescence and in vitro differentiation by 17β-estradiol between mesenchymal stem cells derived from male and female mini-pigs
Won-Jae Lee, Seung-Chan Lee, Jeong-Hyun Lee, Gyu-Jin Rho, Sung-Lim Lee 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 5 159-170 (12 pages)
The characterization and potential of mesenchymal stem cells (MSCs) are gender dependent and estrogen influences these properties. This study demonstrated that supplementation with 17β-estradiol (E2) increases the proliferation of bone marrow-MSCs derived from male and female mini-pigs (Mp- and Fp-BMSCs) in a concentration-dependent manner, with 10 M E2 suggested as the optimal dose of E2 that led to the greatest improvement in BMSCs proliferation. Supplementation of 10 M E2 resulted in... -
Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model
Xiao-ying He, Li-bing Ma, Xiao-ning He, Wan-tong Si, Yue-Mao Zheng 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 3 145-152 (8 pages)
Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated... -
Viscerotropic velogenic Newcastle disease virus replication in feathers of infected chickens
Dong-Hun Lee, Jung-Hoon Kwon, Jin-Yong Noh, Jae-Keun Park, Seong-Su Yuk, Tseren-Ochir Erdene-Ochir, Sang-Soep Nahm, Yong-Kuk Kwo 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 15 115-117 (3 pages)
Newcastle disease viruses (NDVs) cause systemic diseases in chickens with high mortality. However, little is known about persistence of NDVs in contaminated tissues from infected birds. In this study, we examined viral replication in the feather pulp of chickens inoculated with viscerotropic velogenic NDV (vvNDV) genotype VII. Reverse transcription real-time PCR and immunohistochemistry were used to investigate viral persistence in the samples. vvNDV was detected in the oropharynx and cloaca and... -
Establishment and evaluation of a murine ανβ3-integrin- expressing cell line with increased susceptibility to Foot- and-mouth disease virus
Wei Zhang, Kaiqi Lian, Fan Yang, Yang Yang, Zhijian Zhu, Zixiang Zhu, Weijun Cao, Ruoqing Mao, Ye Jin, Jijun He, Jianhong Guo, X 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 3 265-272 (8 pages)
Integrin ανβ3 plays a major role in various signaling pathways, cell apoptosis, and tumor angiogenesis. To examine the functions and roles of ανβ3 integrin, a stable CHO-677 cell line expressing the murine ανβ3 heterodimer (designated as “CHO-677-mανβ3” cells) was established using a highly efficient lentiviral-mediated gene transfer technique. Integrin subunits αν and β3 were detected at the gene and protein levels by polymerase chain reaction (PCR) and indirect... -
Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
Xiaoyu Deng, Yanlong Cong, Renfu Yin, Guilian Yang, Chan Ding, Shengqing Yu, Xiufan Liu, Chunfeng Wang, †, Zhuang Ding, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 7 511-517 (7 pages)
Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two- dimensional electrophoresis (2-DE) and subjected to mass spectrometry analysis. Quantitative intensity analysis showed that the expression of 78... -
A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis
Miguel Salgado*, Cristobal Verdugo, Cord Heuer, Pedro Castillo, Patricia Zamorano 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 9 233-239 (7 pages)
PCR is a highly accurate technique for confirming the presence of Mycobacterium avium subsp. paratuberculosis (Map) in broth culture. In this study, a simple, efficient, and low-cost method of harvesting DNA from Map cultured in liquid medium was developed. The proposed protocol (Universidad Austral de Chile [UACH]) was evaluated by comparing its performance to that of two traditional techniques (a QIAamp DNA Stool Mini Kit and cethyltrimethylammonium bromide [CTAB] method). The results were...
