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371.

Identification of differentially expressed genes in gauze-exposed omentum of dogs using differential display RT-PCR

Md. Mizanur Rahman, Yongsun Kim, Ye-Eun Byeon, Hak-Hyun Ryu, Wan Hee Kim, Mahmuda Umme Rayhan, Oh-Kyeong Kweon* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 8 167-173 (7 pages)

Md. Mizanur Rahman, Yongsun Kim, Ye-Eun Byeon, Hak-Hyun Ryu, Wan Hee Kim, Mahmuda Umme Rayhan, Oh-Kyeong Kweon* 저
...
- 3,000원
Molecular mechanisms governing peritonitis caused by the presence of aseptic gauze have remained unclear. To identify the genes involved, sterile gauze-exposed omentum was collected at 0, 6, 12, 24, and 48 h intervals, and analyzed by differential display RT(reverse transcription)-PCR. Among over 1,200 bands, 230 bands were found differentially expressed. These bands represented the fragment sizes of approximately 200 to 1,500 bp. The eight fragments were expressed differentially in the...
372.

Isolation and characterization of equine amniotic membrane-derived mesenchymal stem cells

Min-Soo Seo, Sang-Bum Park, Hyung-Sik Kim, Jun-gu Kang, Joon-Seok Chae*, Kyung-Sun Kang* 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 6 151-159 (9 pages)

Min-Soo Seo, Sang-Bum Park, Hyung-Sik Kim, Jun-gu Kang, Joon-Seok Chae*, Kyung-Sun Kang* 저
...
- 3,000원
Recent studies have shown that mesenchymal stem cells (MSCs) are able to differentiate into multi-lineage cells such as adipocytes, chondroblasts, and osteoblasts. Amniotic membrane from whole placenta is a good source of stem cells in humans. This membrane can potentially be used for wound healing and corneal surface reconstruction. Moreover, it can be easily obtained after delivery and is usually discarded as classified waste. In the present study, we successfully isolated and characterized...
373.

Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay

Hyuk-Mi Lee, Sung-Ok Song, Sang-Ho Cha, Sung-Bok Wee, Karyn Bischoff, Sung-Won Park, Seong-Wan Son, Hwan-Goo Kang*, Myung-Haing 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 5 143-150 (8 pages)

Hyuk-Mi Lee, Sung-Ok Song, Sang-Ho Cha, Sung-Bok Wee, Karyn Bischoff, Sung-Won Park, Seong-Wan Son, Hwan-Goo Kang*, Myung-Haing 저
...
- 3,000원
Monoclonal antibody (mAb, NVRQS-DON) against deoxynivalenol (DON) was prepared. DON-Ag coated enzyme linked immunosorbent assay (ELISA) and DON-Ab coated ELISA were prepared by coating the DON-BSA and DON mAb. Quantitative DON calculation ranged from 50 to 4,000 ng/mL for DON-Ab coated ELISA and from 25 to 500 ng/mL for DON-Ag coated ELISA. 50% of inhibitory concentration values of DON, HT-2, 15-acetyl-DON, and nivalenol were 23.44, 22,545, 5,518 and 5,976 ng/mL based on the DON-Ab coated ELISA....
374.

Antiviral effect of dietary germanium biotite supplementation in pigs experimentally infected with porcine reproductive and respiratory syndrome virus

Bock-Gie Jung, Jin-A Lee, Bong-Joo Lee* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 4 135-141 (7 pages)

Bock-Gie Jung, Jin-A Lee, Bong-Joo Lee* 저
...
- 3,000원
Germanium biotite (GB) is an aluminosilicate mineral containing 36 ppm germanium. The present study was conducted to better understand the effects of GB on immune responses in a mouse model, and to demonstrate the clearance effects of this mineral against Porcine reproductive and respiratory syndrome virus (PRRSV) in experimentally infected pigs as an initial step towards the development of a feed supplement that would promote immune activity and help prevent diseases. In the mouse model,...
375.

In vitro effects of meloxicam on the number, Foxp3 expression, production of selected cytokines, and apoptosis of bovine CD25+CD4+ and CD25−CD4+ cells

Tomasz Maślanka*, Jerzy Jan Jaroszewski 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 3 125-134 (10 pages)

Tomasz Maślanka*, Jerzy Jan Jaroszewski 저
...
- 3,000원
The purpose of this study was to evaluate the effect of meloxicam (MEL) on selected immune parameters of bovine CD25CD4+, CD25CD4+, and CD25−CD4+ cells. Peripheral blood mononuclear cells (PBMCs) collected from 12-month-old heifers were treated with MEL at a concentration corresponding to the serum level of this medication following administration at the recommended dose (MEL 5 × 10 M) and at a concentration 10 times lower (MEL 5 × 10 M). After 12 and 24 h of incubation with the drug,...
376.

Sporozoite proteome analysis of Cryptosporidium parvum by one-dimensional SDS-PAGE and liquid chromatography tandem mass spectrometry

AMAM Zonaed Siddiki* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 1 107-114 (8 pages)

AMAM Zonaed Siddiki* 저
...
- 3,000원
to explore the stage specific proteome of Cryptosporidium (C.) parvum. To accomplish this, the sporozoite protein of C. parvum was first fractionated using SDS-PAGE with subsequent LC-MS/MS analysis. A total of 135 protein hits were recorded from 20 gel slices (from same gel lane), with many hits occurring in more than one band. Excluding all non-Cryptosporidium entries and proteins with multiple hits, 33 separate C. parvum entries were identified during the study. The overall goal of this study...
377.

Pulsed tissue Doppler imaging of the left ventricular septal mitral annulus in healthy dogs

Jihye Choi, Hyunwook Kim, Junghee Yoon* 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 12 85-90 (6 pages)

Jihye Choi, Hyunwook Kim, Junghee Yoon* 저
...
- 3,000원
This study evaluated pulsed TDI variables including the isovolumic time interval and duration of the major wave in a population of large healthy dogs. Longitudinal myocardial motion at the septal mitral annulus was evaluated with pulsed TDI in 45 healthy adult dogs. Maximal myocardial velocities, isovolumic time intervals, and duration of the myocardial waves were measured. The correlation between time intervals and velocity variables was also investigated. The mean maximal systolic velocity was...
378.

Expression and lytic efficacy assessment of the Staphylococcus aureus phage SA4 lysin gene

Anil Kumar Mishra*, Mayank Rawat, Konasagara Nagaleekar Viswas, Abhishek, Sujeet Kumar, Manjunatha Reddy 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 6 37-43 (7 pages)

Anil Kumar Mishra*, Mayank Rawat, Konasagara Nagaleekar Viswas, Abhishek, Sujeet Kumar, Manjunatha Reddy 저
...
- 3,000원
Treatment of bovine mastitis caused by Staphylococcus (S.) aureus is becoming very difficult due to the emergence of multidrug-resistant strains. Hence, the search for novel therapeutic alternatives has become of great importance. Consequently, bacteriophages and their endolysins have been identified as potential therapeutic alternatives to antibiotic therapy against S. aureus. In the present study, the gene encoding lysin (LysSA4) in S. aureus phage SA4 was cloned and the nucleotide sequence...
379.

Pathological characterization of TgElk mice injected with brain homogenate from elk with chronic wasting disease

Yong-Chul Jeon, Jin-Kyu Choi, Eun-Kyung Choi, Richard I. Carp, Yong-Sun Kim* 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 4 21-26 (6 pages)

Yong-Chul Jeon, Jin-Kyu Choi, Eun-Kyung Choi, Richard I. Carp, Yong-Sun Kim* 저
...
- 3,000원
Chronic wasting disease (CWD) is classified as a transmissible spongiform encephalopathy or prion disease that affects cervids. CWD has been reported in 15 US states, two Canadian provinces, and in imported elk on several farms in Korea. This study was conducted to examine the molecular biological and pathogenic characteristics of a CWD-associated prion isolated in Korea. The epidemiological origin of this pathogen was also determined. Homozygous TgElk mice were infected with a CWD-affected elk...
380.

Quantitation of meloxicam in the plasma of koalas (Phascolarctos cinereus) by improved high performance liquid chromatography

Benjamin Kimble, Kong Ming Li, Merran Govendir* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 2 7-14 (8 pages)

Benjamin Kimble, Kong Ming Li, Merran Govendir* 저
...
- 3,000원
the internal standard (IS) were used for regression analysis of the calibration curve, which was linear from 10 to 1,000 ng/mL (r ＞ 0.9998). Average absolute recovery rates were 91% and 96% for MEL and the IS, respectively. This method had sufficient sensitivity (lower quantitation limit of 10 ng/mL), precision, accuracy, and selectivity for routine analysis of MEL in koala plasma using 250-mL sample volumes. Our technique clearly resolved the MEL peak from the complex koala plasma matrix and...

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