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381.

Immunohistochemical study of osteopontin in boar testis

Seungjoon Kim, Taekyun Shin 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 1 107-110 (4 pages)

Seungjoon Kim, Taekyun Shin 저
...
- 1,500원
The expression of osteopontin (OPN) in boar testis was studied. Western blot analysis detected 66- and 32-kDa OPN immunopositive bands in the testes of adult boars. In postnatal piglets, the 66-kDa OPN band was detected in the testes, but not the 32-kDa band. In the newborn testis, OPN immunostaining was seen in gonocytes and in some supporting cells in the seminiferous tubules, as well as in interstitial Leydig cells. In the adult boar testis, OPN immunoreactivity was detected in seminiferous...
382.

Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos

Kilyoung Song, Eunsong Lee 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 12 81-87 (7 pages)

Kilyoung Song, Eunsong Lee 저
...
- 3,000원
This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) of : 1) co-culturing of oocytes with follicular shell pieces (FSP) during in vitro maturation (IVM); 2) different durations of maturation; and 3) defined maturation medium supplemented with polyvinyl alcohol (PVA; control), pig follicular fluid (pFF), cysteamine (CYS), or β-mercaptoethanol (β-ME). The proportion of metaphase II oocytes was...
383.

Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves

Brajesh C. Varshney, N.M. Ponnanna, Pranati A. Sarkar, Pragna Rehman, Jigar H. Shah 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 9 57-64 (8 pages)

Brajesh C. Varshney, N.M. Ponnanna, Pranati A. Sarkar, Pragna Rehman, Jigar H. Shah 저
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- 3,000원
Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies to K99 antigen and electron microscopy. The K99 antigen from a selected field strain (SAR-14) was heat-extracted and fractionated on a Sepharose CL-4B column. Further purification was carried out by sodium deoxycholate treatment and/or ion-exchange chromatography. Monoclonal antibodies to...
384.

Organotypic slice culture of the hypothalamic paraventricular nucleus of rat

Eun Seong Cho, So Yeong Lee, Jae-Yong Park, Seong-Geun Hong, Pan Dong Ryu 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 3 15-20 (6 pages)

Eun Seong Cho, So Yeong Lee, Jae-Yong Park, Seong-Geun Hong, Pan Dong Ryu 저
...
- 3,000원
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and...
385.

Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens

Ha Jung Roh, Haan Woo Sung, Hyuk Moo Kwon 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 10 361-368 (8 pages)

Ha Jung Roh, Haan Woo Sung, Hyuk Moo Kwon 저
...
- 3,000원
This study examined the adjuvant effects of dimethyl dioctadecyl ammonium bromide (DDA), CpG oligodeoxynucleotides (CpG-ODN), and chicken interferon-γ (ChIFN-γ) on a DNA vaccine (pcDNA-VP243) against the infectious bursal disease virus (IBDV). A plasmid encoding chicken IFN-a was constructed. Twice at 2-week intervals, twoweek- old chickens were injected intramuscularly and intraperitoneally with either a DNA vaccine alone or a DNA vaccine together with the respective adjuvants. On week 2...
386.

Cellular uptake of magnetic nanoparticle is mediated through energy-dependent endocytosis in A549 cells

Jun-Sung Kim, Tae-Jong Yoon, Kyeong-Nam Yu, Mi Suk Noh, Minah Woo, Byung-Geol Kim, Kee-Ho Lee, Byung-Hyuk Sohn, Seung-Bum Park, 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 3 321-326 (6 pages)

Jun-Sung Kim, Tae-Jong Yoon, Kyeong-Nam Yu, Mi Suk Noh, Minah Woo, Byung-Geol Kim, Kee-Ho Lee, Byung-Hyuk Sohn, Seung-Bum Park, 저
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- 3,000원
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery, bioimaging, and biosensors, detailed information of the cellular uptake process of the nanoparticles is essential. Thus, this study was performed to elucidate the precise mechanism by which...
387.

Immunohistochemical localization of calcium binding proteins and some neurotransmitters in myenteric plexus of goat stomach

Heungshik S. Lee, Young Sam Nam 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 2 315-319 (5 pages)

Heungshik S. Lee, Young Sam Nam 저
...
- 1,500원
To understand the neurochemical properties of the gastric myenteric plexus of ruminants, the expression patterns of calbindin D-28k (CB), calretinin (CR), substance P (SP) and calcitonin gene-related peptide (CGRP) were explored in the Korean native goat. In gastric myenteric plexus, CB and SP immunoreactivity were observed in round- or ovalshaped neurons. CR and CGRP immunoreactivity were detected only in the nerve fibers. This immunohistochemical localization of CB, CR, CGRP and SP in the...
388.

Gamma-ray irradiation stimulates the expression of caveolin-1 and GFAP in rat spinal cord: a study of immunoblot and immunohistochemistry

Meejung Ahn, Heechul Kim, Jeong Tae Kim, Jeeyoung Lee, Jin Won Hyun, Jae Woo Park, Taekyun Shin 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 1 309-314 (6 pages)

Meejung Ahn, Heechul Kim, Jeong Tae Kim, Jeeyoung Lee, Jin Won Hyun, Jae Woo Park, Taekyun Shin 저
...
- 3,000원
We studied the expression of caveolin-1 in the spinal cords of rats using 60Co γ-ray irradiation (single dose of 8 Gray (Gy)) in order to determine the possible involvement of caveolin-1 in the tissues of the central nervous system after irradiation. Spinal cords sampled at days 1, 4, and 9 post-irradiation (PI) (n = 5 per each time point) were analyzed by Western blot and immunohistochemistry. Western blot analysis showed that the expression of caveolin-1 was significantly increased at day 1...
389.

Efficacy of VP2 protein expressed in E. coli for protection against highly virulent infectious bursal disease virus

Abdul Rahman Omar, Chong Lee Kim, Mohd Hair Bejo, Aini Ideris 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 6 241-247 (7 pages)

Abdul Rahman Omar, Chong Lee Kim, Mohd Hair Bejo, Aini Ideris 저
...
- 3,000원
The ability of a heat-inactivated whole virus from a highly virulent infectious bursal disease virus (hvIBDV) and VP2 protein from hvIBDV expressed in E. coli provided protection against a hvIBDV challenge in specificpathogen- free (SPF) chickens. Six out of seven chickens that were injected three times with crude VP2 protein developed significant antibody titer against IBDV. However, only four out of the seven chickens survived the hvIBDV challenge. Despite showing low antibody titer profiles,...
390.

Immunohistochemical study of caveolin-1 and -2 in the rat retina

Heechul Kim, Taeki Lee, Jeeyoung Lee, Meejung Ahn, Changjong Moon, Myung Bok Wie, Taekyun Shin 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 1 101-104 (4 pages)

Heechul Kim, Taeki Lee, Jeeyoung Lee, Meejung Ahn, Changjong Moon, Myung Bok Wie, Taekyun Shin 저
...
- 1,500원
The expression of caveolin-1 and -2 in the retina was examined; Western blot analysis showed that both were present. Immunohistochemistry indicated that caveolin-1 was expressed in the majority of retinal layers, including the ganglion cell layer, inner plexiform layer, outer plexiform layer, and in the vascular endothelial cells of the retina. Caveolin-2 was primarily immunostained in the vessels, but in a few other elements as well. This is the first demonstration of caveolin differential...

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