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381.

A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats

Su Hwa Lee, Byeong Yeal Jung, Nabin Rayamahji, Hee Soo Lee, Woo Jin Jeon, Kang Seuk Choi, Chang Hee Kweon, Han Sang Yoo* 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 7 43-51 (9 pages)

Su Hwa Lee, Byeong Yeal Jung, Nabin Rayamahji, Hee Soo Lee, Woo Jin Jeon, Kang Seuk Choi, Chang Hee Kweon, Han Sang Yoo* 저
...
- 3,000원
on sequence data from Genbank. Among the three DNA extraction methods (boiling, alkaline lysis, and QIAamp DNA Mini Kit), the QIAamp DNA Mini Kit was the most sensitive in this study. The optimized multiplex real-time PCR was applied to artificially inoculated beef or pork. The detection sensitivity of the multiplex real-time PCR was increased. The specificity of the multiplex real-time PCR assay, using 128 pure-cultured bacteria including 110 Salmonella isolates and 18 non- Salmonella isolates,...
382.

A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples

Vijayarani Kumanan, Sam R. Nugen, Antje J. Baeumner, Yung-Fu Chang* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 6 35-42 (8 pages)

Vijayarani Kumanan, Sam R. Nugen, Antje J. Baeumner, Yung-Fu Chang* 저
...
- 3,000원
of a small number (ten) of viable Mycobacterium (M.) avium subsp. paratuberculosis (MAP) cells in fecal samples. The assays are based on the identification of the RNA of the IS900 element of MAP. For the assay, RNA was extracted from fecal samples spiked with a known quantity of (10 to 10) MAP cells and amplified using RT-PCR and identified by the LF biosensor and the microtiter plate assay. While the LF biosensor assay requires only 30 min of assay time, the overall process took 10 h for the...
383.

Low numbers of intestinal Shiga toxin-producing E. coli correlate with a poor prognosis in sheep infected with bovine leukemia virus

Witold A. Ferens, Julius Haruna, Rowland Cobbold, Carolyn J. Hovde* 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 6 375-380 (6 pages)

Witold A. Ferens, Julius Haruna, Rowland Cobbold, Carolyn J. Hovde* 저
...
- 3,000원
Healthy ruminants carry intestinal Shiga toxin (Stx)-　producing Escherichia coli (STEC). Stx has antiviral activities in vitro and STEC numbers correlate with reduced early viremia in sheep experimentally infected with bovine leukemia virus (BLV). This study assessed the impact of intestinal STEC on BLV-induced disease for one year post-BLV-challenge. High STEC scores (CFU/g feces × frequency of STEC-positive samples) correlated with good health, whereas poor weight gain, distress, and...
384.

In vitro and in vivo gene therapy with CMV vector-mediated presumed dog β-nerve growth factor in pyridoxine-induced neuropathy dogs

Jin-Young Chung, Jung-Hoon Choi, Il-Seob Shin, Eun-Wha Choi, Cheol-Yong Hwang, Sang-Koo Lee, Hwa-Young Youn* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 5 367-374 (8 pages)

Jin-Young Chung, Jung-Hoon Choi, Il-Seob Shin, Eun-Wha Choi, Cheol-Yong Hwang, Sang-Koo Lee, Hwa-Young Youn* 저
...
- 3,000원
Due to the therapeutic potential of gene therapy for neuronal injury, many studies of neurotrophic factors, vectors, and animal models have been performed. The presumed dog β-nerve growth factor (pdβ-NGF) was generated and cloned and its expression was confirmed in CHO cells. The recombinant pdβ-NGF protein reacted with a human β-NGF antibody and showed bioactivity in PC12 cells. The pdβb-NGF was shown to have similar bioactivity to the dog β-NGF. The recombinant pdβ-NGF plasmid was...
385.

Protection of chickens from Newcastle disease with a recombinant baculovirus subunit vaccine expressing the fusion and hemagglutinin- neuraminidase proteins

Youn-Jeong Lee, Haan-Woo Sung, Jun-Gu Choi, Eun-Kyoung Lee, Hachung Yoon, Jae-Hong Kim, Chang-Seon Song 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 11 301-308 (8 pages)

Youn-Jeong Lee, Haan-Woo Sung, Jun-Gu Choi, Eun-Kyoung Lee, Hachung Yoon, Jae-Hong Kim, Chang-Seon Song 저
...
- 3,000원
Recombinant baculoviruses containing the fusion (F) and hemagglutinin-neuraminidase (HN) glycoprotein gene of the viscerotropic velogenic (vv) Newcastle disease virus (NDV) isolate, Kr-005/00, and a lentogenic La Sota strain of the NDV were constructed in an attempt to develop an effective subunit vaccine to the recent epizootic vvNDV. The level of protection was determined by evaluating the clinical signs, mortality, and virus shedding from the oropharynx and cloaca of chickens after a...
386.

Plasma haptoglobin and immunoglobulins as diagnostic indicators of deoxynivalenol intoxication

Eun-Joo Kim, Sang-Hee Jeong, Joon-Hyoung Cho, Hyun-Ok Ku, Hyun-Mi Pyo, Hwan-Goo Kang, Kyoung-Ho Choi 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 5 257-266 (10 pages)

Eun-Joo Kim, Sang-Hee Jeong, Joon-Hyoung Cho, Hyun-Ok Ku, Hyun-Mi Pyo, Hwan-Goo Kang, Kyoung-Ho Choi 저
...
- 3,000원
This study aimed to discover potential biomarkers for dioxynivalenol (DON) intoxication. B6C3F1 male mice were orally exposed to 0.83, 2.5 and 7.5 mg/kg body weight (bw) DON for 8 days and the differential protein expressions in their blood plasma were determined by SELDI - Time-of-Flight/ Mass Spectrometry (TOF/MS) and the immunoglobulins (Igs) G, A, M and E in the serum were investigated. 11.7 kDa protein was significantly highly expressed according to DON administration and this protein was...
387.

All blood, No stool: enterohemorrhagic Escherichia coli O157:H7 infection

Jang W. Yoon, Carolyn J. Hovde 대한수의학회 Journal of Veterinary Science 14 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 1 219-232 (14 pages)

Jang W. Yoon, Carolyn J. Hovde 저
...
- 4,300원
Enterohemorrhagic Escherichia coli serotype O157:H7 is a pathotype of diarrheagenic E. coli that produces one or more Shiga toxins, forms a characteristic histopathology described as attaching and effacing lesions, and possesses the large virulence plasmid pO157. The bacterium is recognized worldwide, especially in developed countries, as an emerging food-borne bacterial pathogen, which causes disease in humans and in some animals. Healthy cattle are the principal and natural reservoir of E....
388.

Aquaporin 1 expression in tissues of canines possessing inherited high K erythrocytes

Hideharu Ochiai*, Nobuya Hishiyama, Shin Hisamatsu, Nobuyuki Kanemaki 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 12 203-206 (4 pages)

Hideharu Ochiai*, Nobuya Hishiyama, Shin Hisamatsu, Nobuyuki Kanemaki 저
...
- 1,500원
We investigated the expression of aquaporin 1 (AQP1) in tissues from canines with an inherited anomaly that causes their erythrocytes to have high K. Northern blot analysis revealed abundant AQP1 expression in lung and kidney, though little expression was found in spleen. Using anti- C-terminus for dog AQP1, abundant expression was shown in kidney, trachea, and eye, but little expression was shown in pancreas and cerebrum, indicating that AQP1 expression in canine tissues is similar to that...
389.

The C-terminal variable domain of LigB from Leptospira mediates binding to fibronectin

Yi-Pin Lin, Yung-Fu Chang* 대한수의학회 Journal of Veterinary Science 12 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 3 133-144 (12 pages)

Yi-Pin Lin, Yung-Fu Chang* 저
...
- 4,100원
a significant reduction in binding of the variable region of LigB to Fn using small interfering RNA (siRNA). Finally, the isothermal titration calorimetric results confirmed the interaction between the variable region of LigB and Fn. This is the first report to demonstrate that LigB binds to MDCK cells. In addition, the reduction of Fn expression in the MDCK cells, by siRNA, reduced the binding of LigB. Taken together, the data from the present study showed that LigB is a Fn-binding protein of...
390.

Molecular characterization and genogrouping of VP1 of aquatic birnavirus GC1 isolated from rockfish Sebastes schlegeli in Korea

Seong Joon Joh, *, Chae Ik Shon, Sung Won Kang, Byoung Han Kim, Byung Yul Jeong, Kyung Gi Lee, Jun Hun Kwon, Gang Jun Heo2 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 11 85-90 (6 pages)

Seong Joon Joh, *, Chae Ik Shon, Sung Won Kang, Byoung Han Kim, Byung Yul Jeong, Kyung Gi Lee, Jun Hun Kwon, Gang Jun Heo2 저
...
- 3,000원
The cDNA nucleotide sequence of genome segment B encoding the VP1 protein was determined for the aquatic birnavirus GC1 isolated from the rockfish Sebastes schlegeli in Korea. The VP1 protein of GC1 contains a 2,538 bp open reading frame, which encodes a protein comprising 846 amino acid residues that has a predicted MW of 94 kDa. The sequence contains 6 potential Asn-X-Ser/Thr motifs. Eight potential Ser phosphorylation sites and 1 potential Tyr phophorylation site were also identified. GC1...

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