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Inoculation of Lewis lung carcinoma cells enhances formalin-induced pain behavior and spinal Fos expression in mice
Jae-Gyun Choi, Jae-Min Kim, Dong-Wook Kang, Jung-Wan Choi, Jin Bong Park, Seong-Hun Ahn, Yeonhee Ryu, Hyun-Woo Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 2 267-272 (6 pages)
The incidence of lung cancer has rapidly increased and cancer patients at a later cancer stage frequently suffer from unbearable cancer-associated pain. However, the pathophysiology of lung cancer pain has not been fully described due to a lack of appropriate animal models. This study was designed to determine the effect of Lewis lung carcinoma (LLC) cell inoculation on formalin-induced pain behavior and spinal Fos expression in C57BL/6 mice. LLC cells (1.5 × 10, 2.5 × 10, 3.0 × 10 or 5.0 ×... -
Molecular and phylogenetic analysis of Anaplasma spp. in sheep and goats from six provinces of China
Yan Zhang, Yali Lv, Feifei Zhang, Wenjing Zhang, Jinhong Wang, Yanyan Cui, Rongjun Wang, Fuchun Jian, Longxian Zhang, Changshen 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 11 523-529 (7 pages)
Members of the genus Anaplasma are important emerging tick-borne pathogens in both humans and animals in tropical and subtropical areas. Here, we investigated the presence of Anaplasma spp. in 621 sheep and 710 goats from six provinces of China. Polymerase chain reaction (PCR) and DNA sequencing were conducted to determine the prevalence of Anaplasma (A.) phagocytophilum, A. ovis and A. bovis targeting the 16S ribosomal RNA or the major surface protein 4 gene. PCR revealed Anaplasma in 39.0%... -
Toxicity of crude and detoxified Tityus serrulatus venom in anti-venom-producing sheep
Marina G. Ferreira, Clara G. Duarte, Maira S. Oliveira, Karen L. P. Castro, Ma?lson S. Teixeira, L?lian P. G. Reis, Jos? A. Zamb 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 5 467-477 (11 pages)
Specific anti-venom used to treat scorpion envenomation is usually obtained from horses after hyperimmunization with crude scorpion venom. However, immunized animals often become ill because of the toxic effects of the immunogens used. This study was conducted to evaluate the toxic and immunogenic activities of crude and detoxified Tityus serrulatus (Ts) venom in sheep during the production of anti-scorpionic anti-venom. Sheep were categorized into three groups: G1, control, immunized with... -
Experimental infection and pathology of clade 2.2 H5N1 virus in gulls
Marina A. Gulyaeva, Kirill A. Sharshov, Anna V. Zaykovskaia, Lidia V. Shestopalova, Aleksander M. Shestopalov 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 7 179-188 (10 pages)
During 2006, H5N1 HPAI caused an epizootic in wild birds, resulting in a die-off of Laridae in the Novosibirsk region at Chany Lake. In the present study, we infected common gulls (Larus canus) with a high dose of the H5N1 HPAI virus isolated from a common gull to determine if severe disease could be induced over the 28 day experimental period. Moderate clinical signs including diarrhea, conjunctivitis, respiratory distress and neurological signs were observed in virus-inoculated birds, and 50%... -
Characterisation of genotype VII Newcastle disease virus (NDV) isolated from NDV vaccinated chickens, and the efficacy of LaSota and recombinant genotype VII vaccines against challenge with velogenic NDV
Kiarash Roohani, Sheau Wei Tan, Swee Keong Yeap, Aini Ideris, Mohd Hair Bejo, Abdul Rahman Omar 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 8 447-457 (11 pages)
A Newcastle disease virus (NDV) isolate designated IBS002 was isolated from a commercial broiler farm in Malaysia. The virus was characterised as a virulent strain based on the multiple basic amino acid motif of the fusion (F) cleavage site RRRKGF and length of the C-terminus extension of the hemagglutinin-neuraminidase (HN) gene. Furthermore, IBS002 was classified as a velogenic NDV with mean death time (MDT) of 51.2 h and intracerebral pathogenicity index (ICPI) of 1.76. A genetic distance... -
Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis
Xiao-wei Zhao, Yong-xin Yang, Dong-wei Huang, Guang-long Cheng, Hui-ling Zhao 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 2 253-263 (11 pages)
severe clinical symptoms, including damage to mammary tissues, reduced milk yield, and altered milk composition. In order to investigate the host response to E. coli infection and discover novel markers for mastitis treatment, mammary tissue samples were collected from healthy cows and bovines with naturally occurring severe E. coli mastitis. Changes of mammary tissue proteins were examined using two-dimensional gel electrophoresis and label-free proteomic approaches. A total... -
Tarantula cubensis extract alters the degree of apoptosis and mitosis in canine mammary adenocarcinomas
Nilgun Gultiken, Tolga Guvenc, Duygu Kaya, Ali Reha Agaoglu, Serhan Serhat Ay, Ibrahim Kucukaslan, Birten Emre, Murat Findik, Sa 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 11 213-219 (7 pages)
In the present study, 13 clinical cases of canine mammary adenocarcinoma were evaluated in order to understand the effect of Tarantula cubensis extract (TCE) on tumor tissue. Punch biopsies were taken from the tumors before treatment with TCE. Subcutaneous injections of TCE were administered three times at weekly intervals (3 mL per dog). Between days 7 and 10 after the third injection, the tumor masses were extirpated by complete unilateral mastectomy. Pre- and post-treatment tumor tissues were... -
Quantitative real-time polymerase chain reaction for detecting Mycoplasma hyosynoviae and Mycoplasma hyorhinis in pen-based oral, tonsillar, and nasal fluids
João Carlos Gomes Neto, Leslie Bower, Barbara Z. Erickson, Chong Wang, Matthew Raymond, Erin L. Strait 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 9 195-201 (7 pages)
Mycoplasma (M.) hyorhinis and M. hyosynoviae are pathogens known to cause disease in pigs post-weaning. Due to their fastidious nature, there is increased need for culture-independent diagnostic platforms to detect these microorganisms. Therefore, this study was performed to develop and optimize quantitative real-time PCR (qPCR) assays to rapidly detect M. hyorhinis and M. hyosynoviae in pen-based oral fluids as well as nasal and tonsillar fluids as proxies for samples used in swine herd... -
Comparison of three skin-stretching devices for closing skin defects on the limbs of dogs
Vassiliki Tsioli, Lysimachos G. Papazoglou, Nikolaos Papaioannou, Dimitra Psalla, Ioannis Savvas, Leonidas Pavlidis, Ma 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 13 99-106 (8 pages)
Our objective was to evaluate the effectiveness of skin-stretching devices for closing defects on the extremities of dogs. Antebrachial skin defects were created on the limbs of 24 dogs randomly divided into three groups. Skin stretchers included staples and sutures passing through them (group A), sutures and hypodermic needles (group B), and Pavletic device (group C). Wounds on the left were further undermined in all groups. Tension and blood perfusion were assessed.... -
Use of hydrophilic extra-viral domain of canine distemper virus H protein for enzyme-linked immunosorbent assay development
Ki-hyun Cho, Jeongmi Kim, Hyun-ah Yoo, Dae-hee Kim, Seung-yong Park, Chang-seon Song, In-soo Choi, Joong-bok Lee, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 6 503-509 (7 pages)
Simple methods for measuring the levels of serum antibody against canine distemper virus (CDV) would assist in the effective vaccination of dogs. To develop an enzyme-linked immunosorbent assay (ELISA) specific for CDV, we expressed hydrophilic extra-viral domain (HEVD) protein of the A75/17-CDV H gene in a pET 28a plasmid-based Escherichia (E.) coli vector system. Expression was confirmed by dot and Western blotting. We proposed that detection of E. coli-expressed H protein might be...


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