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An inactivated vaccine to control the current H9N2 low pathogenic avian influenza in Korea
Jun Gu Choi, Youn Jeong Lee, *, Yong Joo Kim, Eun Kyoung Lee, Ok Mi Jeong, Haan Woo Sung, Jae Hong Kim, Jun Hun Kwon1 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 9 67-74 (8 pages)
The H9N2 subtype low pathogenic avian influenza is one of the most prevalent avian diseases worldwide, and was first documented in 1996 in Korea. This disease caused serious economic loss in Korea's poultry industry. In order to develop an oil-based inactivated vaccine, a virus that had been isolated in 2001 (A/chicken/Korea/01310/ 2001) was selected based on its pathogenic, antigenic, and genetic properties. However, in animal experiments, the efficacy of the vaccine was found to be very low... -
The combination of deoxynivalenol and zearalenone at permitted feed concentrations causes serious physiological effects in young pigs
Feng Chen, Yulin Ma, Chunyi Xue, Jingyun Ma, Qingmei Xie, Genhu Wang, Yingzuo Bi, Yongchang Cao, * 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 6 39-44 (6 pages)
This study was to investigate the effects of the combination of deoxynivalenol (DON) and zearalenone (ZON) on pigs. Twenty-four weaning piglets were divided into a control group fed a diet free of mycotoxins and a toxin group fed a diet containing 1 mg/kg DON and 250 Ռg/kg ZON. The results showed that supplementation of DON and ZON in diets had extensive effects on pigs. More specifically, DON and ZON caused levels of total protein, albumin, and globulin in sera to decrease (p < 0.05) by... -
Effects of endocrine disrupting chemicals on expression of phospholipid hydroperoxide glutathione peroxidase mRNA in rat testes
In-Jeoung Baek, Jung-Min Yon, Se-Ra Lee, Yan Jin, Mi-Ra Kim, Byeongwoo Ahn, Jin Tae Hong, Young-Kug Choo, Beom Jun Lee, Young Wo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 2 213-218 (6 pages)
Phospholipid hydroperoxide glutathione peroxidase (PHGPx), an antioxidative selenoprotein, is modulated by estrogen in the testis and oviduct. To examine whether potential endocrine disrupting chemicals (EDCs) affect the microenvironment of the testes, the expression patterns of PHGPx mRNA and histological changes were analyzed in 5-week-old Sprague-Dawley male rats exposed to several EDCs such as an androgenic compound [testosterone (50, 200, and 1,000 ?g/kg)], anti-androgenic compounds... -
Biological characteristics of Chinese hamster ovary cells transfected with bovine Prnp
Sang-Gyun Kang, Deog-Yong Lee, Mi Lan Kang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 5 131-137 (7 pages)
of transfectants was 10 times higher than that of CHO-K1 cells at 6 h after treatment (p < 0.05). The genomic DNA of both the transfectants and control cells began to be fragmented at 6 h after treatment with cyclohexamide. Caspase-3 activity was reduced by transfection with the bovine Prnp (p < 0.05). Conclusively, the viability of transfectants expressing exogenous bovine Prnp was decreased while the capacities for cellular protection against antioxidative stress and apoptosis were increased. -
Detection of swine hepatitis E virus in the porcine hepatic lesion in Jeju Island
Song-Hak Lee, Sang-Chul Kang, Dae-Yong Kim, Jong-Hee Bae, Jae-Hoon Kim 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 8 51-55 (5 pages)
Swine hepatitis E virus (HEV) is an emerging zoonotic pathogen due to its close genomic similarity to human HEV. The prevalence of swine HEV in the hepatic lesion of pigs from the Jeju Island was investigated by reverse transcriptase polymerase chain reaction (RT-PCR). In total, 40 pigs with hepatitis lesions were selected from 19 different farms, based on examination by microscopy. RTPCR findings revealed swine HEV in 22 cases (55%), including 18 suckling pigs and 4 growing pigs. Several... -
Sero-survey on Aino, Akabane, Chuzan, bovine ephemeral fever and Japanese encephalitis virus of cattle and swine in Korea
Seong In Lim, Chang Hee Kweon, Dong Seob Tark, Seong Hee Kim, Dong Kun Yang 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 7 45-49 (5 pages)
Vector-borne arboviruses produce mild to severe symptoms in domestic animals. Bovine ephemeral fever (BEF), Akabane, Aino, and Chuzan virus have been primarily attributed to reproductive disorders or febrile diseases in cattle, and Japanese encephalitis virus (JEV) is mainly associated with reproductive failures in swine. We investigated antibody titers from domestic swine against four bovine arboviruses (BEF, Akabane, Aino, and Chuzan virus) and from cattle against JEV in Korea. While the... -
Detection of betanodaviruses in apparently healthy aquarium fishes and invertebrates
Dennis Kaw Gomez, Dong Joo Lim, Gun Wook Baeck, Hee Jeong Youn, Nam Shik Shin, Hwa Young Youn, Cheol Yong Hwang, Jun Hong Park, 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 11 369-374 (6 pages)
Betanodaviruses are the causative agents of viral nervous necrosis (VNN) in cultured marine fish. A total of 237 apparently healthy aquarium fish, marine (65 species) and freshwater (12 species) fishes and marine invertebrates (4 species), which were stocked in a commercial aquarium in Seoul, South Korea, were collected from November 2005 to February 2006. The brains of the fish and other tissues of the invertebrates were examined by reverse transcriptasepolymerase chain reaction (RT-PCR) and... -
Characterization of HC58cDNA, a putative cysteine protease from the parasite Haemonchus contortus
Charles I. Muleke, Yan Ruofeng, Xu Lixin, Sun Yanming, Li Xiangrui 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 7 249-255 (7 pages)
Because of the complexity of the cathepsin B-like (CBL) family, an information on the biological and biochemical characteristics of individual CBL genes is lacking. In this study, we investigated the degradative effects of the recombinant HC58 protein isolated from Haemonchus contortus parasites on protein substrates over a broad pH range in vitro. This protein, which hydrolyzed the synthetic peptide substrates Z-FR-AMC and Z-RR-AMC, had characteristics of the cysteine protease class of... -
Characterization of Chinook head salmon embryo phenotypes of infectious salmon anemia virus by real-time RT-PCR
Khalid Munir 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 13 167-176 (10 pages)
effects (CPE) in the CHSE-214 cell line, viral titration of the infected CHSE-cell harvests, and analysis of viral RNA levels in CHSE-214 cells at post-infection day zero, 7 and 14 by SYBR Green real-time RT-PCR confirmed the existence of three CHSE-phenotypes of ISAV: replicating cytopathic, replicating non-cytopathic, and non-replicating non-cytopathic. The identification of these three CHSE- phenotypes of ISAV has important implications from diagnostic and biological points of view. -
Development of immunoassays for the detection of kanamycin in veterinary fields
Yong Jin, Jin-Wook Jang, Chang-Hoon Han, Mun-Han Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 3 111-117 (7 pages)
Monoclonal antibody against kanamycin was prepared, and competitive direct ELISA and immunochromatographic assay were developed using the antibody to detect kanamycin in animal plasma and milk. The monoclonal antibody produced was identified to be IgG1, which has a kappa light chain. No cross-reactivity of the antibody was detected with other aminoglycosides, indicating that the monoclonal antibody was highly specific for kanamycin. Based on competitive direct ELISA, the detection limits of...


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