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전체 선택해제
총 1,307건 검색
본문검색 결과만 보기
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Effects of High Glucose on Na,K-ATPase and Na/glucose Cotransporter Activity in Primary Rabbit Kidney Proximal Tubule Cells
Han, Ho-Jae 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 1호 7 69-80 (12 pages)
transport systems. Primary cultures of rabbit proximal tubules, which achieved confluence at 10 days, exhibited brush-border characteristics typical of proximal tubular cells. Northern analysis indicated 2.2 ~ 2.3 and 2.0 kb Na/glucose cotransporter RNA species appeared in fresh and cultured proximal tubule cells after confluence, repectively. The cultured cells showed reduced Na/glucose cotransporter activity compared to fresh proximal tubules. Primary cultured proximal tubule cells incubated i... -
RNA Modification and Its Implication in Plant Pathogenic Fungi
Junhyun Jeon, Song Hee Lee 한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 6호 1 505-511 (7 pages)
layer for genetic information, cul- minating in epitranscriptome concept. In plant patho- genic fungi, however, the presence and the biological roles of RNA modifications are largely unknown. Here we delineate types of RNA modifications, and provide examples demonstrating roles of such modifications in biology of filamentous fungi including fungal patho- gens. We also discuss the possibility that RNA modifica- tion systems in fungal pathogens could be a prospective target for new agrochemicals. -
Identification of Plant Viruses Infecting Pear Using RNA Sequencing
Nam-Yeon Kim, Hyo-Jeong Lee, Hong-Sup Kim, Su-Heon Lee, Jae-Sun Moon, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 5 258-267 (10 pages)
overview of virus populations infecting a major pear cultivar (‘Singo’) in Korea. From June 2017 to October 2019, leaf samples (n = 110) of pear trees from 35 orchards in five major pear-producing regions were collected and subjected to RNA sequencing. Most virus-associated contigs matched the sequences of known viruses, including apple stem grooving virus (ASGV) and apple stem pit- ting virus (ASPV). However, some contigs matched the sequences of apple green crinkle-associated virus and... -
DNA Microarray and Gene Ontology Enrichment Analysis Reveals That a Mutation in opsX Affects Virulence and Chemotaxis in Xanthomonas oryzae pv. oryzae
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 3호 3 190-200 (11 pages)
a saccharide biosynthesis regulatory protein, on the virulence and bacterial chemotaxis of Xoo. We performed DNA microarray analysis, which showed that 63 of 2,678 genes, including genes related to bacterial motility (flagellar and chemotaxis proteins) were significantly downregulated (<−2 log;sub>2;/sub> fold changes) by the mutation in opsX. Indeed, motility assays showed that the mutant strain was nonmotile on semisolid agar swarm plates. In addition, a mutant strain... -
Identification of Coupling and Repulsion Phase DNA Marker Associated With an Allele of a Gene Conferring Host Plant Resistance to Pigeonpea sterility mosaic virus (PPSMV) in Pigeonpea (Cajanus cajan L. Millsp.)
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 1호 4 33-40 (8 pages)
a set of 32 out of 300 short decamer random DNA markers that showed polymorphism between Gullyal white and BSMR 736 parents. Among them, eleven DNA markers showed polymorphism including coupling and repulsion phase type of polymorphism across the parents. Bulked Segregant Analysis (BSA), revealed that the DNA marker, IABTPPN7, produced a single coupling phase marker (IABTPPN7;sub>414;/sub>) and a repulsion phase marker (IABTPPN7;sub>983;/sub>) co-segregating with PSMD reaction.... -
RT-PCR Detection of Five Quarantine Plant RNA Viruses Belonging to Potyand Tospoviruses
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 3호 12 291-296 (6 pages)
developed reverse transcription-polymerase chain reaction (RT-PCR) primer pairs for five single-stranded (ss) plant RNA viruses that are not currently reported in Korea but could be potential harmful plant viral pathogens. Three viruses such as Chilli veinal mottle virus (ChiVMV), Colombian datura virus (CDV), and Tobacco etch virus (TEV) belong to the genus Potyvirus while Chrysanthemum stem necrosis virus (CSNV) and Iris yellow spot virus (IYSV) are members of the genus Tospovirus. To design... -
The Road to RNA Silencing is Paved with Plant-Virus Interactions
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 3호 1 197-206 (10 pages)
had a large impact on biology in general, as well as on our understanding of plantpathogen interactions, especially interactions between plants and viruses. While most of what we know about the mechanism of RNA silencing was deduced in the last 12 years, many of the interactions between plants and viruses, as well as virus-virus interactions in plants, which we now know are manifestations of RNA silencing, were the subject of decades of work from numerous laboratories. These laboratories were... -
Genetic Reassortment of Rice stripe virus RNA Segments Detected by RT-PCR Restriction Enzyme Analysis-based Method
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 7 148-155 (8 pages)
a clear pattern of RNA reassortment, i.e., different groups of isolates having their RNA segments derived from two to three different RSV ancestors, such as from Eastern and Southwestern Chinese or Japanese M and T isolates. We also found that the migratory small brown planthopper from Eastern China caught by aerial net traps that possesses RSVRNA3 genotypes corresponds mainly to Eastern China, with a few for Southwestern China based on RT-PCR/ RE, sequence and phylogenetic analyses, indicating... -
Simple and Reliable DNA Extraction Method for the Dark Pigmented Fungus, Cercospora sojina
한국식물병리학회 The Plant Pathology Journal 4 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 15 289-292 (4 pages)
the plant pathogenic fungus Cercospora sojina. Total DNA yield obtained by this method was approximately 1 mg/g of mycelia (fresh weight), and the mean ratio of A260/A280 and A260/A230 were 2.04 and 2.1, respectively. The results of random amplified polymorphic DNA (RAPD) analysis, digestion with restriction enzymes, and Southern hybridization indicated that polysaccharides were effectively removed by this method, and the resulting DNA was sufficient for use in subsequent molecular analysis. -
Microwave Irradiation-assisted RNA Extraction from Woody Tissues for Plant Virus Detection
한국식물병리학회 The Plant Pathology Journal 3 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 14 286-288 (3 pages)
method, however, is both time-consuming and expensive. Additionally, homogenization may generate excessive endogenous RNases, polyphenolics, and other substances that reduce the quality and quantity of RNA. In this study, we describe the microwave irradiation-assisted RNA extraction (MIRE) technique which, without tissue disruption and homogenization, allows for the cost-effective and rapid generation of intact RNA from apple cane shavings and the reliable detection of apple virus by RT-PCR.
