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Electroretinography recordings using a light emitting diode active corneal electrode in healthy beagle dogs
Yoshiki Itoh, Seiya Maehara, Norihiko Itoh*, Kazuto Yamashita, Yasuharu Izumisawa 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 11 77-84 (8 pages)
Electroretinography (ERG) is a well-established diagnostic procedure for objectively evaluating retinal function. In this study, ERG in beagle dogs, which are a popular experimental animal, was performed to determine the normal range of ERG variables and assess differences between the left and right eyes. ERG findings including rod, combined rod-cone, single-flash cone, and 30-Hz flicker responses were recorded with an LED-electrode in 43 sedated beagle dogs. The subjects were divided into young... -
Effects of conditioned media from human amniotic epithelial cells on corneal alkali injuries in rabbits
Tae-Hyun Kim, Young-Woo Park, Jae-Sang Ahn, Jeong-Taek Ahn, Se-Eun Kim, Man-Bok Jeong, Min-Su Seo, Kyung-Sun Kang, Kang-Moon Seo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 9 61-67 (7 pages)
This study was performed to evaluate the effects of conditioned media (CM) from human amniotic epithelial cells (HAECs) on the corneal wound healing process. Eighteen rabbits (36 eyes) were used and randomly assigned to three groups according treatment: CM from HAECs (group 1), vehicle alone (group 2), and saline (group 3). Corneal alkali injuries were induced with 1 N sodium hydroxide. Each reagent used for treatment evaluation was injected into the dorsal bulbar subconjunctiva and the area of... -
Protection of chickens against infectious bronchitis virus with a multivalent DNA vaccine and boosting with an inactivated vaccine
Fang Yan*, Yujun Zhao, Yongting Hu, Jianyang Qiu, Wenxin Lei, Wenhui Ji, Xuying Li, Qian Wu, Xiumin Shi, Zhong Li 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 8 53-60 (8 pages)
The protective efficacy of DNA plasmids encoding avian infectious bronchitis virus (IBV) S1, N, or M protein was investigated in chickens. Chickens were inoculated monovalently (with plasmid pVAX1-16S1, pVAX1-16M, or pVAX1-16N alone) or multivalently (combination of the three different plasmids, pVAX1-16S1/M/N). A prime-boost immunization protocol against IBV was developed. Chickens were immunized with the multivalent DNA vaccine twice and then boosted with an inactivated vaccine once. Antibody... -
Trends in genotype frequency resulting from breeding for resistance to classical scrapie in Belgium (2006∼2011)
Alexandre Dobly*, Sara Van der Heyden, Stefan Roels 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 7 45-51 (7 pages)
In sheep, susceptibility to scrapie is mainly determined by codons 136, 154, and 171 of the PRNP gene. Five haplotypes are usually present (ARR, ARQ, ARH, AHQ, and VRQ). The ARR haplotype confers the greatest resistance to classical scrapie while VRQ renders animals most susceptible. In 2004, the European Union implemented a breeding program that promotes selection of the ARR haplotype while reducing the incidence of VRQ. From 2006 to 2011 in Belgium, frequency for the ARR/ARR genotypes... -
Molecular characterization of Japanese encephalitis virus strains prevalent in Chinese swine herds
Hao Zheng, Tongling Shan, Yu Deng, Chunqing Sun, Shishan Yuan, Yang Yin, Guangzhi Tong* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 5 27-36 (10 pages)
Japanese encephalitis virus (JEV) is the leading cause of viral encephalitis in Asia and domestic pigs serve as the amplifying hosts. In the present study, the full genomic sequences of two JEV strains (HEN0701 and SH0601) isolated from pigs in China were determined and compared with other 12 JEV strains deposited in GenBank. These two strains had an 88.8% nucleotide sequence similarity and 97.9% deduced amino acid sequence homology. HEN0701 had high nucleotide sequence and high amino acid... -
Pathological characterization of TgElk mice injected with brain homogenate from elk with chronic wasting disease
Yong-Chul Jeon, Jin-Kyu Choi, Eun-Kyung Choi, Richard I. Carp, Yong-Sun Kim* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 4 21-26 (6 pages)
Chronic wasting disease (CWD) is classified as a transmissible spongiform encephalopathy or prion disease that affects cervids. CWD has been reported in 15 US states, two Canadian provinces, and in imported elk on several farms in Korea. This study was conducted to examine the molecular biological and pathogenic characteristics of a CWD-associated prion isolated in Korea. The epidemiological origin of this pathogen was also determined. Homozygous TgElk mice were infected with a CWD-affected elk... -
Quantitation of meloxicam in the plasma of koalas (Phascolarctos cinereus) by improved high performance liquid chromatography
Benjamin Kimble, Kong Ming Li, Merran Govendir* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 2 7-14 (8 pages)
× 3.9 mm) column. Retention times for MEL and piroxicam were 8.03 and 5.56 min, respectively. Peak area ratios of MEL to the internal standard (IS) were used for regression analysis of the calibration curve, which was linear from 10 to 1,000 ng/mL (r > 0.9998). Average absolute recovery rates were 91% and 96% for MEL and the IS, respectively. This method had sufficient sensitivity (lower quantitation limit of 10 ng/mL), precision, accuracy, and selectivity for routine analysis of MEL in koala... -
C6, a new monoclonal antibody, reacts with the follicle-associated epithelium of calf ileal Peyer's patches
Kana Tozaki, Junpei Kimura, Masahiro Yasuda*, Nobuyuki Ryu, Testuo Nasu, Anton Pernthaner, Wayne R. Hein 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 1 1-6 (6 pages)
The follicle-associated epithelium (FAE) of Peyer's patches (PPs) contains M cells that are important for reducing mucosal immune responses by transporting antigens into the underlying lymphoid tissue. We generated a monoclonal antibody (C6) that reacted with the FAE of calf ileal PPs, and analyzed the characteristics of C6 using immunohistochemistry and Western blotting. FAE of the ileal PP was stained with C6 during both late fetal developmental and postnatal stages. Neither the villous... -
Detection of Torque teno sus virus 1 and 2 in tissues from stillborn piglets delivered by sows via natural farrowing
Chenga Tshering, Mitsuhiro Takagi, Eisaburo Deguchi* 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 13 425-427 (3 pages)
chain reaction. The detection rates of TTSuV1 and TTSuV2 were 78% and 50%, respectively, with 83% of the stillborn piglets positive for TTSuV1 or TTSuV2. TTSuV1 was detected highest in the liver (72%) followed by heart (56%), spleen (38%) and tonsils (38%) while TTSuV2 was detected highest in the tonsils (38%) followed by liver (33%), spleen (25%), and heart (17%). These results indicate that TTSuVs are commonly present but not equally distributed among the tissues of stillborn piglets. -
Suitability of autologous serum for expanding rabbit adipose-derived stem cell populations
Jae-Jun Bahn, Jin-Young Chung, Wooseok Im, Manho Kim, Sae Hoon Kim* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 11 413-417 (5 pages)
Adipose-derived stem cells (ASCs) are believed to have potential use for treating many illnesses. Most cells, including ASCs, are generally cultured in medium containing fetal bovine serum (FBS). However, FBS, which could induce an immune response or infection, is not recommended for clinical applications. In the present study, we evaluated the morphology, proliferation rate, and characterization of rabbit ASCs grown in medium containing autologous serum (AS) and compared these cells to ones...


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