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561.

Pathotyping avian pathogenic Escherichia coli strains in Korea

Yong-Wun Jeong, Tae-Eun Kim, Jae-Hong Kim*, Hyuk-Joon Kwon* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 5 145-152 (8 pages)

Yong-Wun Jeong, Tae-Eun Kim, Jae-Hong Kim*, Hyuk-Joon Kwon* 저
...
- 3,000원
the strains were classified into 27 molecular pathotypes (MPs). The MP25, MP19, and MP10 pathotypes possessingiroN-fimC-ompT-hlyF-iucD-tsh-iss-irp2-fyuA (22.8%), iroN-fimC-ompT-hlyF-iucD-tsh-iss (21.8%), and iroN-fimC-ompT-hlyF-iss (11.9%) genotypes, respectively, were predominant. Redundancy of iron uptake-related genes was clearly observed and some strains were associated with higher mortality than others. Therefore, strains with the predominant genotypes can be used for diagnosis and vaccine.
562.

Sequence variations of the bovine prion protein gene (PRNP) in native Korean Hanwoo cattle

Sangho Choi, Hee-Jong Woo*, Jeongmin Lee* 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 3 127-137 (11 pages)

Sangho Choi, Hee-Jong Woo*, Jeongmin Lee* 저
...
- 4,000원
Bovine spongiform encephalopathy (BSE) is one of the fatal neurodegenerative diseases known as transmissible spongiform encephalopathies (TSEs) caused by infectious prion proteins. Genetic variations correlated with susceptibility or resistance to TSE in humans and sheep have not been reported for bovine strains including those from Holstein, Jersey, and Japanese Black cattle. Here, we investigated bovine prion protein gene (PRNP) variations in Hanwoo cattle [Bos (B.) taurus coreanae], a native...
563.

Production of a highly group-specific monoclonal antibody against zearalenone and its application in an enzyme-linked immunosorbent assay

Sang-Ho Cha, Sung-Hee Kim, Karyn Bischoff, Hyun-Jeong Kim, Seong-Wan Son, Hwan-Goo Kang* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 2 119-125 (7 pages)

Sang-Ho Cha, Sung-Hee Kim, Karyn Bischoff, Hyun-Jeong Kim, Seong-Wan Son, Hwan-Goo Kang* 저
...
- 3,000원
two direct competitive ELISA systems using the selected antibody (ZEN-coated and anti-ZEN antibody-coated ELISA). Quantitative ranges for the anti-ZEN antibody-coated ELISA and ZEN-coated ELISA were from 25 to 750 ppb and from 12.5 to 100 ppb, respectively. The detection limit of both methods as measured with standard solutions was 10 ppb. The intra-plate and inter-well variation of both ELISAs were less than 10%. The IC50 values for a-zearalenol, b-zearalenol, a-zearalanol, and b-zearalanol...
564.

Prevalence and antibiotic resistance of mastitis pathogens isolated from dairy herds transitioning to organic management

Young Kyung Park, Lawrence K. Fox*, Dale D. Hancock, Wade McMahan, Yong Ho Park* 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 15 103-105 (3 pages)

Young Kyung Park, Lawrence K. Fox*, Dale D. Hancock, Wade McMahan, Yong Ho Park* 저
...
- 1,500원
Changes in udder health and antibiotic resistance of mastitis pathogens isolated from dairies upon conversion from conventional to organic management over a 3-year period was studied. Coagulase-negative staphylococci (CNS) were the most prevalent mastitis pathogens isolated. CNS were significantly less resistant to b-lactam antibiotics when isolated from milk after the herd transitioned to organic management. Cessation of the use of antimicrobial therapies in dairies in combination with organic...
565.

Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis

Siju Joseph*, Naicy Thomas, E. Thangapandian, Vijendra P Singh, Rishendra Verma, S. K. Srivastava 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 14 99-101 (3 pages)

Siju Joseph*, Naicy Thomas, E. Thangapandian, Vijendra P Singh, Rishendra Verma, S. K. Srivastava 저
...
- 1,500원
A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based...
566.

Establishment and characterization of an infectious cDNA clone of a classical swine fever virus LOM strain

Gil-Soon Park, Seong-In Lim, Seung-Ho Hong, Jae-Young Song* 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 12 81-91 (11 pages)

Gil-Soon Park, Seong-In Lim, Seung-Ho Hong, Jae-Young Song* 저
...
- 4,000원
Classical swine fever virus (CSFV) causes a highly contagious disease among swine that has an important economic impact worldwide. CSFV strain LOM is an attenuated virus of low virulent strain of Miyagi isolated from Japan in 1956. Eight DNA fragments representing the genome of the CSFV strain LOM were obtained by RT-PCR. These were used to determine the complete nucleotide sequence and construct a full-length cDNA clone which was called Flc-LOM. Sequence analysis of the recombinant clone...
567.

Development and characterization of a potential diagnostic monoclonal antibody against capsid protein VP1 of the chicken anemia virus

Yi-Yang Lien, Chi-Hung Huang, Fang-Chun Sun, Shyang-Chwen Sheu, Tsung-Chi Lu, Meng-Shiunn Lee, Shu-Chin Hsueh, Hsi-Jien Chen, Me 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 11 73-79 (7 pages)

Yi-Yang Lien, Chi-Hung Huang, Fang-Chun Sun, Shyang-Chwen Sheu, Tsung-Chi Lu, Meng-Shiunn Lee, Shu-Chin Hsueh, Hsi-Jien Chen, Me 저
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- 3,000원
Chicken anemia virus (CAV) is an important viral pathogen that causes anemia and severe immunodeficiency syndrome in chickens worldwide. In this study, a potential diagnostic monoclonal antibody against the CAV VP1 protein was developed which can precisely recognize the CAV antigen for diagnostic and virus recovery purposes. The VP1 gene of CAV encoding the N-terminus-deleted VP1 protein, VP1Nd129, was cloned into an Escherichia (E.) coli expression vector. After...
568.

Effect of oxytocin infusion on luteal blood flow and progesterone secretion in dairy cattle

Christos N. Brozos, Metin S. Pancarci, Javier Valencia, Nikola Beindorff, Georgios Tsousis*, Evaggelos Kiossis, Heinrich Bollwei 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 10 67-71 (5 pages)

Christos N. Brozos, Metin S. Pancarci, Javier Valencia, Nikola Beindorff, Georgios Tsousis*, Evaggelos Kiossis, Heinrich Bollwei 저
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- 1,500원
The objective of this study was to investigate the effects of oxytocin infusion on corpus luteum (CL) function during early to mid-diestrus by measuring luteal size (LS) and luteal blood flow (LBF) along with plasma levels of progesterone (P4) and prostaglandin metabolites (13,14-dihydro-15-keto-prostaglandin F2a, PGFM). On day (D) 7 of the estrus cycle (D1 = ovulation), seven cows received 100 IU of oxytocin (OXY) or placebo (PL) following a Latin square design. LS and LBF increased in both...
569.

Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors

Andrea Maria Blanc, Mabel Beatriz Berois, Lorena Magalí Tomé, Alberto L. Epstein, Juan Ramón Arbiza* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 9 59-65 (7 pages)

Andrea Maria Blanc, Mabel Beatriz Berois, Lorena Magalí Tomé, Alberto L. Epstein, Juan Ramón Arbiza* 저
...
- 3,000원
Herpes simplex virus type-1 (HSV-1) amplicon vectors are versatile and useful tools for transferring genes into cells that are capable of stimulating a specific immune response to their expressed antigens. In this work, two HSV-1-derived amplicon vectors were generated. One of these expressed the full-length glycoprotein D (gD) of bovine herpesvirus 1 while the second expressed the truncated form of gD (gDtr) which lacked the trans-membrane region. After evaluating gD expression in the infected...
570.

Comparison of four diagnostic methods for detecting rabies viruses circulating in Korea

Dong-Kun Yang*, Eun-Kyung Shin, Yoon-I Oh, Kyung-Woo Lee, Chung-San Lee, Seo-Young Kim, Jeong-A Lee, Jae-Young Song 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 7 43-48 (6 pages)

Dong-Kun Yang*, Eun-Kyung Shin, Yoon-I Oh, Kyung-Woo Lee, Chung-San Lee, Seo-Young Kim, Jeong-A Lee, Jae-Young Song 저
...
- 3,000원
for rabies according to the FAT. Compared to the FAT, the sensitivities of VI, RT-PCR, and RIDA were 100, 100, and 95%, respectively. The specificities of VI, RT-PCR and RIDA were found to be 100, 100, and 98.9%, respectively. Rabies viruses circulating in Korea were isolated and propagated in murine neuroblastoma (NG108-15) cells with titers ranging from 10 to 10 TCID50/mL. Although the RIDA findings did not completely coincide with results obtained from FAT, VI, and RT-PCR, RIDA appears to be...

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