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Genotype analysis of Cryptosporidium spp. prevalent in a rural village in Hwasun-gun, Republic of Korea
PARK. Jae-Hwan, GUK. Sang-Mee, HAN. Eun-Taek, SHIN. Eun-Hee, KIM. Jae-Lip, CHAI. Jong-Yil 대한기생충학회 The Korean journal of parasitology 7 Pages
대한기생충학회 The Korean journal of parasitology 2006, Vol.44 No.1 27-33 (7 pages)
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Monitoring of Horizontal Gene Transfer from Agricultural Microorganisms to Soil Bacteria and Analysis of Microbial Community in Soils
Kim. Sung-Eun, Moon. Jae-Sun, Choi. Won-Sik, Lee. Sang-Han, Kim. Sung-Uk 한국미생물생명공학회 Journal of microbiology and biotechnology 4 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2012, Vol.22 No.4 563-566 (4 pages)
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Differential diagnosis of Salmonella gallinarum and S. pullorum using PCR-RFLP
Myeong Kyu Park, Kyoung Seong Choi, Myeong Chul Kim, Joon Seok Chae 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 3호 9 213-219 (7 pages)
nucleotide sequence analysis. The homology of nucleotide sequence was 99.7% between S. gallinarum and S. pullorum rfbS genes. Further comparisons of the sequences of S. gallinarum, S. gallinarum fied strain, S. pullorum and S. typhi(GenBank Accession No.M29682) showed a homology of 98.3%. The predicted amino acid sequence homology was 97.1%, 97.1% and 97.5%, respectively. Based on this comparison of these nucleotide sequences, we found unique restriction enzyme sites within the rfbS genes of S.... -
Phenotypic and genotypic analyses of an attenuated porcine reproductive and respiratory syndrome virus strain after serial passages in cultured porcine alveolar macrophages
Seung-Chul Lee, Sunhee Lee, Gun-Woo Yoo, Hwan-Won Choi, Yun-Hee Noh, Chang Eon Park, Jae-Ho Shin, In-Joong Yoon, Shien-Young Kang4, Chang 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 5 358-367 (10 pages)
The porcine reproductive and respiratory syndrome virus (PRRSV) is a globally ubiquitous swine viral pathogen that causes major economic losses worldwide. We previously reported an over-attenuated phenotype of cell-adapted PRRSV strain CA-2-P100 in vivo. In the present study, CA-2-P100 was serially propagated in cultured porcine alveolar macrophage (PAM) cells for up to 20 passages to obtain the derivative strain CA-2-MP120. Animal inoculation studies revealed that both CA-2-P100 and CA-2-MP120... -
A new recombined porcine reproductive and respiratory syndrome virus virulent strain in China
Jian-guo Dong, Lin-yang Yu, Pei-pei Wang, Le-yi Zhang, Yan-ling Liu, Peng-shuai Liang, Chang-xu Song 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 12 89-98 (10 pages)
high morbidity, and high mortality in sows and piglets. The genome of this new strain was 15,413 nucleotides (nt) long, and comparative analysis revealed that GDsg shared 82.4% to 94% identity with type 2 PRRSV strains, but only 61.5% identity with type 1 PRRSV Lelystad virus strain. Phylogenetic analysis indicated that type 2 PRRSV isolates include five subgenotypes (I, II, III, IV, and V), which are represented by NADC30, VR-2332, GM2, CH-1a, and HuN4, respectively. Moreover, GDsg belongs to a... -
Antibiotic resistance and molecular characterization of ophthalmic Staphylococcus pseudintermedius isolates from dogs
Min-Hee Kang, Min-Joo Chae, Jang-Won Yoon, Seung-Gon Kim, So-Young Lee, Jong-Hyun Yoo, Hee-Myung Park* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 9 409-415 (7 pages)
and surveyed for species-specific sequences in the Staphylococcus intermedius group (SIG) nuclease gene (SInuc), exfoliative toxin gene for SIG (siet), and antibiotic resistance genes (blaZ and mecA). PCR-restriction fragment length polymorphism analysis of the pta gene was also performed. Fifty isolates were identified as SIG strains, all of which were found to be SP. The blaZ gene was detected in 42 of the 50 SP strains and mecA gene was observed in 18 of the 50 SP strains. The 50 SP strains... -
Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle
Hee-Jin Dong, Ae-Ri Cho, Tae-Wook Hahn, Seongbeom Cho* 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 19 317-325 (9 pages)
A multiplex loop-mediated isothermal amplification (mLAMP) assay was developed for simultaneous detection of the stx1 and stx2 genes and applied for detection of shiga toxin-producing Escherichia coli (STEC) in cattle farm samples. Two target genes were distinguished based on Tm values of 85.03 ± 0.54C for stx1 and 87.47 ± 0.35C for stx2. The mLAMP assay was specific (100% inclusivity and exclusivity), sensitive (with a detection limit as low as 10 fg/μL), and quantifiable (R = 0.9313). The...
