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Comparison of the age-related porcine endogenous retrovirus (PERV) expression using duplex RT-PCR
Hyoung Joon Moon, Hye Kwon Kim, Seong Jun Park, Chul Seung Lee, Dae Sub Song, Bo Kyu Kang, Bong Kyun Park* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 7 317-322 (6 pages)
Porcine endogenous retroviruses (PERVs) are members of family Retroviridae, genus Gamma retrovirus, and transmitted by both horizontally and vertically like other endogenous retroviruses (ERVs). PERV was initially described in the 1970s having inserted its gene in the host genome of different pig breeds, and three classes, PERV-A, PERV-B, and PERV-C are known. The therapeutic use of living cells, tissues, and organs from animals called xenotransplantation might relieve the limited supply of... -
Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms
Hye Kwon Kim, Jeong Sun Yang, Hyoung Joon Moon, Seong Jun Park, Yuzi Luo, Chul Seung Lee, Dae Sub Song, Bo Kyu Kang, Soo Kyung A 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 5 121-130 (10 pages)
The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic... -
Serosurveillance for Japanese encephalitis, Akabane, and Aino viruses for Thoroughbred horses in Korea
Dong-Kun Yang*, Byoung-han Kim, Chang-Hee Kweon, Jin-Ju Nah, Hyun-Joo Kim, Kyung-Woo Lee, Young-Jin Yang, Kyu-Whan Mun 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 7 381-386 (6 pages)
Recent global warming trends may have a significant impact on vector-borne viral diseases, possibly affecting vector population dynamics and disease transmission. This study measured levels of hemagglutination-inhibition (HI) antibodies against Japanese encephalitis virus (JEV) and neutralizing antibodies against Akabane virus (AKAV) and Aino virus (AINV) for Thoroughbred horses in Korea. Blood samples were collected from 989 racehorses in several provinces, between October 2005 and March 2007.... -
The seroprevalence of Japanese encephalitis virus in goats raised in Korea
Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, In-Jin Hwang, Mun-Il Kang, Byung-Jae So, Kyoung-Oh Cho 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 16 197-199 (3 pages)
Japanese encephalitis virus (JEV) causes a mosquitoborne viral zoonosis that is becoming increasingly important to public health in east and south Asia. Although JEV is primarily associated with reproductive failure in swine, JEV infection can cause fever and headache in humans and is associated with aseptic meningitis and encephalitis. The exact mode of transmission, including host range and possible source of viral amplification within livestock, is still not completely clear. This study... -
Detection of swine hepatitis E virus in the porcine hepatic lesion in Jeju Island
Song-Hak Lee, Sang-Chul Kang, Dae-Yong Kim, Jong-Hee Bae, Jae-Hoon Kim 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 8 51-55 (5 pages)
by microscopy. RTPCR findings revealed swine HEV in 22 cases (55%), including 18 suckling pigs and 4 growing pigs. Several histopathological lesions, including multifocal lymphoplasmacytic hepatitis, portal inflammation, and focal hepatocellular necrosis, were observed in liver sections of swine HEV PCR-positive pigs. The present study suggests that the prevalence of swine HEV is very high in the pig population in Jeju Island, and that pigs are infected at early stages of growth (under 2 months... -
Sero-survey on Aino, Akabane, Chuzan, bovine ephemeral fever and Japanese encephalitis virus of cattle and swine in Korea
Seong In Lim, Chang Hee Kweon, Dong Seob Tark, Seong Hee Kim, Dong Kun Yang 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 7 45-49 (5 pages)
Vector-borne arboviruses produce mild to severe symptoms in domestic animals. Bovine ephemeral fever (BEF), Akabane, Aino, and Chuzan virus have been primarily attributed to reproductive disorders or febrile diseases in cattle, and Japanese encephalitis virus (JEV) is mainly associated with reproductive failures in swine. We investigated antibody titers from domestic swine against four bovine arboviruses (BEF, Akabane, Aino, and Chuzan virus) and from cattle against JEV in Korea. While the... -
Development and evaluation of indirect ELISA for the detection of antibodies against Japanese encephalitis virus in swine
Dong-Kun Yang, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Kyung-Woo Lee, Cheong-Up Choi, Chang-Hee Kweon 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 10 271-275 (5 pages)
The Japanese encephalitis virus (JEV) is one of causative agents of reproductive failure in pregnant sows. An indirect enzyme-linked immunosorbent assay (I-ELISA) was examined for its potential use in the rapid monitoring of the JEV, and the results were compared with those from the hemagglutination inhibition (HI) and serum neutralization (SN) tests. The comparative analysis showed that the results of I-ELISA showed a significant correlation with the conventional HI (r = 0.867) and SN tests (r... -
Comparison of an enzyme-linked immunosorbent assay with serum neutralization test for serodiagnosis of porcine epidemic diarrhea virus infection
Jin Sik Oh, Dae Sub Song, Jeong Sun Yang, Ju Young Song, Hyoung Joon Moon, Tae Yung Kim, Bong Kyun 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 14 349-352 (4 pages)
An indirect porcine epidemic diarrhea (PED)virus (PEDV)enzyme-linked immunosorbent assay (ELISA) was compared with the serum neutralization (SN)test by testing 46 samples from experimentally infected sows,73 samples from naive sows,and 1,024 field sow samples from 48 commercial swine farms of undefined PED status. The SN test and the ELISA were performed using PEDV, KPEDV-9 strain.Viral proteins as a coating antigen of PEDV ELISA were extracted from the cytoplasm of PEDV-infected Vero cells... -
Immunogenicity of baculovirus expressed recombinant proteins of Japanese encephalitis virus in mice
Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, Seong-In Lim, Jun-Hun Kwon, Seong-Hee Kim, Jae-Young Song, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 6 125-133 (9 pages)
Genes encoding for the premembrane and envelope (prME), envelope (E) and nonstructural protein (NS1) of Japanese encephalitis virus (JEV) were cloned. Each protein was expressed in baculovirus expression system. Of the three proteins expressed in baculovirus system, only prME had hemagglutination activity. The prME (72 and 54 kDa), E (54 kDa) and NS1 (46 kDa) proteins could be detected by Western blotting in the recombinant virus infected cells. Immunogenicity of the recombinant proteins... -
Effect of soluble porcine aminopeptidase N on antibody production against porcine epidemic diarrhea virus
Jin Sik Oh, Dae Sub Song, Jeong Sun Yang, Ju Young Song, Han Sang Yoo, Yong Suk Jang, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 11 353-357 (5 pages)
A few members of coronavirus group I which includes porcine epidemic diarrhea virus (PEDV) use porcine aminopeptidase N (pAPN) as a cellular receptor. Cellular receptors play an important role in virus attachment and entry. However, the low permissiveness of PEDV to APN-expressing porcine cell lines has made it difficult to elucidate the role of pAPN in vitro. The purpose of this study was to prove whether the treatment of soluble pAPN could enhance the antibody production against PEDV in guinea...


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