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효모 ABF1 단백질의 DNA Binding 부위에 대한 구조 기능 연구
조기남, 이상경, 김홍태, 김지영, 노현모, 전구홍, Cho. Gi-Nam, Lee. Sang-Kyung, Kim. Hong-Tae, Kim. Ji-Young, Rho. Hyune-Mo, Jung. Gu-Hung 한국미생물학회 미생물학회지 7 Pages
한국미생물학회 미생물학회지 1994, Vol.32 No.2 102-108 (7 pages)
ABF1(Autonomously replicating sequence Binding Factor 1)은 효모 genome에서 $RTCRYN_5ACG$의 염기 서열을 가지고 있는 promoter, mating-type silencer, ARS에 결합하는 DNA binding 단백질이다. E. coli 에서 ABF1 유전자를 발현하기 위하여, ABF1 유전자를 pMAL-c2 벡터에 cloning하였다.(pMAHW). pMAHW를 E. coli에 형질전환하여, ABF1 융합단백질을 발현시키고, amylose resin affinity chromatography에 의하여 분리하였다. Factor Xa protease를 이용하여 분리된 융합단백질로부터 maltose binding protein을 잘라낸 후에 gel... -
Electrochemical determination of chloramphenicol using a glassy carbon electrode modified with dendrite-like Fe₃O₄ nanoparticles
Krishnan Giribabu, Sung-Chan Jang, Yuvaraj Haldorai, Muruganantham Rethinasabapathy, Seo Yeong Oh, Arunkumar Rengaraj, Young-Kyu Han 한국탄소학회 Carbon Letters 10 Pages
한국탄소학회 Carbon Letters 2017, Vol.23 6 38-47 (10 pages)
In this study, magnetite (Fe3O4) nanoparticles were electrochemically synthesized in an aqueous electrolyte at a given potential of -1.3 V for 180 s. Scanning electron microscopy revealed that dendrite-like Fe3O4 nanoparticles with a mean size of < 80 nm were electrodeposited on a glassy carbon electrode (GCE). The Fe3O4/GCE was utilized for sensing chloramphenicol (CAP) by cyclic voltammetry and square wave voltammetry. A reduction peak of CAP at the Fe3O4/GCE was observed at 0.62 V, whereas... -
Nitrogen-doped carbon nanosheets from polyurethane foams and removal of Cr(VI)
Jiaqi Duan, Baohua Zhang, Huailin Fan, Wenzhong Shen, Shijie Qu 한국탄소학회 Carbon Letters 10 Pages
한국탄소학회 Carbon Letters 2017, Vol.22 7 60-69 (10 pages)
Nitrogen-doped carbon nanosheets with a developed porous structure were prepared from polyurethane foams by hydrothermal carbonization following ZnCl2 chemical activation. Scanning electron microscopy, thermogravimetric analysis, Fourier transform infrared spectroscopy, solid state 13C nuclear magnetic resonance (NMR) spectra and X-ray photoelectron spectroscopy were used to characterize the nitrogen-doped carbon nanosheet structure and composition. The removal of Cr(VI) by the N-doped carbon... -
In silico analysis of putative drug and vaccine targets of the metabolic pathways of Actinobacillus pleuropneumoniae using a subtractive/comparative genomics approach
Biruk T. Birhanu, Seung-Jin Lee, Na-Hye Park, Ju-Beom Song, Seung-Chun Park 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 5 188-199 (12 pages)
Actinobacillus pleuropneumoniae is a Gram-negative bacterium that resides in the respiratory tract of pigs and causes porcine respiratory disease complex, which leads to significant losses in the pig industry worldwide. The incidence of drug resistance in this bacterium is increasing; thus, identifying new protein/gene targets for drug and vaccine development is critical. In this study, we used an in silico approach, utilizing several databases including the Kyoto Encyclopedia of Genes and... -
Heat Shock Proteins: A Review of the Molecular Chaperones for Plant Immunity
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 4호 1 323-333 (11 pages)
As sessile organisms, plants are exposed to persistently changing stresses and have to be able to interpret and respond to them. The stresses, drought, salinity, chemicals, cold and hot temperatures, and various pathogen attacks have interconnected effects on plants, resulting in the disruption of protein homeostasis. Maintenance of proteins in their functional native conformations and preventing aggregation of non-native proteins are important for cell survival under stress. Heat shock proteins... -
Actin Cytoskeleton and Golgi Involvement in Barley stripe mosaic virus Movement and Cell Wall Localization of Triple Gene Block Proteins
한국식물병리학회 The Plant Pathology Journal 14 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 1호 3 17-30 (14 pages)
Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene block (TGB) proteins were coexpressed in cells with the actin marker DsRed: Talin. TGB ectopic expression experiments revealed that TGB3 is a major elicitor of filament thickening, that TGB2 resulted in formation of intermediate DsRed:Talin... -
Roles of Plant Proteases in Pathogen Defense
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 4호 1 367-374 (8 pages)
The genomes of plants contain more than 600 genes encoding a diverse set of proteases and the subunits of proteasomes. These proteases and proteasomes consist of plant proteolytic systems, which are involved in various cellular metabolic processes. Plant proteolytic systems have been shown to have diverse roles in defense responses, such as execution of the attack on the invading organisms, participation in signaling cascades, and perception of the invaders. In order to provide a framework for... -
Triple isotope-[13C, 15N, 2H] labeling and NMR measurements of the inactive, reduced monomer form of Escherichia coli Hsp33
Lee. Yoo-Sup, Ko. Hyun-Suk, Ryu. Kyoung-Seok, Jeon. Young-Ho, Won. Hyung-Sik 한국자기공명학회 Journal of the Korean magnetic resonance society 10 Pages
한국자기공명학회 Journal of the Korean magnetic resonance society 2010, Vol.14 No.2 117-126 (10 pages)
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Overexpression and Characterization of Vibrio mimicus Metalloprotease
Shin. Seung-Yeol, Lee. Jong-Hee, Huh. Sung-Hoi, Park. Young-Seo, Kim. Jin-Man, Kong. In-Soo 한국미생물생명공학회 Journal of microbiology and biotechnology 8 Pages
한국미생물생명공학회 Journal of microbiology and biotechnology 2000, Vol.10 No.5 612-619 (8 pages)
