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수집모유의 미생물오염과 항균제 내성에 관한 연구
황경미, 강영실 한국기초간호학회 Journal of Korean Biological Nursing Science 10 Pages
한국기초간호학회 Journal of Korean Biological Nursing Science 2008, Vol.10 No.2 4 131-140 (10 pages)
모유는 영양학적인 우수성과 위생학적인 안전성, 그리고 모체로부터의 선천적인 면역성분과 모자 간의 신뢰감을 형성 하는 정서적인 측면에서 영유아들에게 필수적으로 공급되어 야 하는 식품이다. 이러한 모유의 장점 때문에 최근 모유 먹이 기 운동이 확산되어 가고 있고 정상 신생아뿐만 아니라 고위 험군 환아에게도 모유를 공급하려는 경향이 있다(Kim, Kim, An, Bae, & Kim, 2003). 고위험군 환아 및 미숙아에게 모유를 수유시키고자 하는 경 우에는 산모의 손이나 유축기를 사용하여 착유된 모유(expressed human milk)를 먹이게... -
Digital Literacy in the 2015 and 2022 Revised Science Curriculum: Emphasis on Elementary Science
Lim Heejun 경인교육대학교 교육연구원 교육논총 12 Pages
경인교육대학교 교육연구원 교육논총 제 42권 특별호 6 67-78 (12 pages)
This study compared the characteristics of digital literacy in the 2022 revised science curriculum to the 2015 revised science curriculum for elementary school. In the aspects of the overview, achievement standards, teaching-learning and evaluation method in 2015 and 2022 revised science curriculum documents, the content related to digital literacy was compared. The 2015 revised curriculum offered at the level use of audio-visual tools or Internet data. The 2022 revised curriculum, in contrast,... -
Enhancing effects of serum-rich and cytokine-supplemented culture conditions on developing blastocysts and deriving porcine parthenogenetic embryonic stem cells
Soo-Kyung Jung, Hyun-Jung Kim, Chan-Lan Kim, Joo-Hyeong Lee, Jin-Young You, Eun-Song Lee, Jeong-Mook Lim, Seon Jong Yun, Jae-You 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 9 519-528 (10 pages)
by strong enzymatic alkaline phosphatase activity and the expression of pluripotent markers OCT3/4, Nanog, and SSEA4. Moreover, the ppESCs were induced to form an embryoid body and teratoma. Differentiation into three germ layers (ectoderm, mesoderm, and endoderm) was confirmed by the expression of specific markers for the layers and histological analysis. In conclusion, data from the present study suggested that our modified culture conditions using FBS and cytokines are highly useful for... -
Root Colonization and ISR-mediated Anthracnose Disease Control in Cucumber by Strain Enterobacter asburiae B1
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2009, 25권 4호 5 333-343 (11 pages)
of strain B1 and a chemical inducer benzothiadiazole (BTH) to tobacco plants induced GUS activity which is linked with activation of PR promoter gene. Furthermore, in Arabidopsis thaliana plants the soil drench of strain B1 induced the defense gene expression of PR1 and PDF1.2 related to salicylic acid and jasmonic acid/ethylene signaling pathways, respectively. In this study, for the main focus on root colonization by strain B1 associated with defense responses, bacterial cells of strain B1 was... -
Stability of pUC-Derived Plasmids with a Fluorescence Marker in Pectobacterium carotovorum subsp. carotovorum and subsp. betavasculorum
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2009, 25권 3호 13 286-290 (5 pages)
carotovorum Pcc21. However, temperature change significantly affected plasmid stability on both Pectobacterium strains. Almost all strains lost the plasmids with the shift in temperature from 28oC to 37oC. Presence of the plasmids did not affect bacterial pathogenicity on their own host plants. Among three plasmids, pZsYellow was not useful as a marker because the yellow fluorescent proteins from pZsYellow were interfered with the yellow natural fluorescence of the plant tissues induced by the... -
Characterization of a Novel Necrotic Response of Glycine max Line ``PI96188`` toXanthomonas axonopodis pv. glycines
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2007, 23권 3호 9 193-202 (10 pages)
at 2 days after inoculation. While photosynthesisrelated rbcS gene showed the dramatic change in Jinjoo1, this gene was constitutively expressed in PI96188. However, expression of the defense-related genes, such as peroxidase and isoflavone synthase in the infected PI96188 leaves was similar to that in Jinjoo1. Together, these results suggest that the novel necrotic symptom in PI96188 is a kind of resistant response different from a typical hypersensitive response in the resistant genotypes. -
Indentification of Differentially Displayed Genes of a Pseudomonas Resistant Soybean(Glycine max)
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2003, 19권 5호 5 239-247 (9 pages)
to a virulent bacterial strain of a Pseudomonas sp. SN239. Specific genes involved in the resistance of the soybean genotype 561 were identified and the pattern of gene expression against the Pseudomonas infection was analyzed using differential-display reverse transcription PCR (DDRTPCR). More than 126 cDNA fragments representing mRNAs were induced within 48 hours of bacteria inoculation. Among them, 28 cDNA fragments were cloned and sequenced. Twelve differentially displayed clones with open... -
Toward Functional Genomics of Plant-Pathogen Interactions: Isolation and Analysis of Defense-related Genes of Hot Pepper Expressed During Resistance Against Pathogen
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2002, 18권 2호 2 63-67 (5 pages)
micro-arrayed for further analysis. Random EST sequence database were also generated from various cDNA libraries including pepper tissue specific libraries and leaves showing non-host hypersensitive response against X. campestris pv. glycines. As a primary stage, thousands of cDNA clones were sequenced and EST data were analyzed. These clones are being spotted on glass slide to study the expression profiling. Results of this study may further broaden knowledge on plant-pathogen interactions. -
Production and On-column Re-folding of Human Vascular Endothelial Growth Factor 165 in Escherichia coli
Bang. Sun Kwon, Kim. Young Sik, Chang. Byung Soo, Park. Cheol Beom, Bang. In Seok 한국생물공학회 Biotechnology and bioprocess engineering 8 Pages
한국생물공학회 Biotechnology and bioprocess engineering 2013, Vol.18 No.5 835-842 (8 pages)
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Site-Specific Recombination by the Integrase MJ1 on Mammalian Cell
김혜영, 윤보현, 장효일, Kim. Hye-Young, Yoon. Bo-Hyun, Chang. Hyo-Ihl 한국미생물생명공학회 한국미생물·생명공학회지 8 Pages
한국미생물생명공학회 한국미생물·생명공학회지 2011, Vol.39 No.4 337-344 (8 pages)
보이기 위해 attP와 attB를 가지고 있는 vector를 MJ1 expression vector와 함께 NIH3T3 cell에 cotransfection 했으며 GFP를 reporter로 사용해 그 activity를 관찰하였다. NIH3T3 cell에서 GFP의 발현을 형광 현미경을 통해 알아본 결과, MJ1에 의한 sitespecific integration이 다른 accessory protein의 도움 없이 일어난다는 것을 볼 수 있었다. 마찬가지 방법으로, attR과 attL 간의 excision을 GFP로 알아본 결과, GFP는 발현하지 않았으며, 이는 MJ1에 의한 excision이 일어나지 않았음을 보여주었다. 이와 같은 결과로 볼 때,...


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