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721.

DevelDevelopment of a Quantitative Real-time Nucleic Acid Sequence based Amplification (NASBA) Assay for Early Detection of Apple scar skin viroid-time Nucleic Acid Sequence based Amplification (NASBA) Assay for Early Detection of Apple scar skin viroid

Seong Heo, Hyun Ran Kim, Hee Jae Lee 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 8 164-171 (8 pages)

Seong Heo, Hyun Ran Kim, Hee Jae Lee 저
...
- 3,000원
An assay for detecting Apple scar skin viroid (ASSVd) was developed based on nucleic acid sequence based amplification (NASBA) in combination with realtime detection during the amplification process using molecular beacon. The ASSVd specific primers for amplification of the viroid RNA and molecular beacon for detecting the viroid were designed based on highly conserved regions of several ASSVd sequences including Korean isolate. The assay had a detection range of 1 × 104 to 1 × 1012 ASSVd RNA...
722.

Molecular Identification and Evaluation of Indigenous Bacterial Isolates for Their Plant Growth Promoting and Biological Control Activities against Fusarium Wilt Pathogen of Tomato

Amanul Islam, Md. Shahinur Kabir, Abul Khair 한국식물병리학회 The Plant Pathology Journal 12 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 5 137-148 (12 pages)

Amanul Islam, Md. Shahinur Kabir, Abul Khair 저
...
- 4,100원
In search of an effective biological control agent against the tomato pathogen Fusarium oxysporum f. sp. lycopersici, rhizospheric soil samples were collected from eight agro-ecological zones of Bangladesh. Among the bacteria isolated from soil, 24 isolates were randomly selected and evaluated for their antagonistic activity against F. oxysporum f. sp. lycopersici. The two promising antagonistic isolates were identified as Brevundimonas olei and Bacillus methylotrophicus based on morphological,...
723.

Factors Affecting the Vineyard Populational Diversity of Plasmopara viticola

Susana Boso, Pilar Gago, Jos?-Luis Santiago, Mar?a de la Fuente, Mar?a-Carmen Mart?nez 한국식물병리학회 The Plant Pathology Journal 12 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 4 125-136 (12 pages)

Susana Boso, Pilar Gago, Jos?-Luis Santiago, Mar?a de la Fuente, Mar?a-Carmen Mart?nez 저
...
- 4,100원
Vitis vinifera is very susceptible to downy mildew (Plasmopara viticola). A number of authors have suggested different genetic populations of this fungus exist in Europe, each showing a different degree of virulence. Work performed to date indicates this diversity to be the result of different factors. In areas where gene flow is greater and recombination more frequent, the diversity of P. viticola appears to be wider. In vineyards isolated by geographic barriers, a race may become dominant and...
724.

Ascospore Infection and Colletotrichum Species Causing Glomerella Leaf Spot of Apple in Uruguay

Sandra Alaniz, Vanessa Cuozzo, Valentina Mart?nez, Marciel J. Stadnik, Pedro Mondino 한국식물병리학회 The Plant Pathology Journal 12 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 2호 2 100-111 (12 pages)

Sandra Alaniz, Vanessa Cuozzo, Valentina Mart?nez, Marciel J. Stadnik, Pedro Mondino 저
...
- 4,100원
Glomerella leaf spot (GLS) caused by Colletotrichum spp. is a destructive disease of apple restricted to a few regions worldwide. The distribution and evolution of GLS symptoms were observed for two years in Uruguay. The recurrent ascopore production on leaves and the widespread randomized distribution of symptoms throughout trees and orchard, suggest that ascospores play an important role in the disease dispersion. The ability of ascospores to produce typical GLS symptom was demonstrated by...
725.

Rapid Detection of SdhBP225F and SdhBH272R Mutations in Boscalid Resistant Botrytis cinerea Strains by ARMS-PCR

Xin Liu, Rong Zeng, Shigang Gao, Lihui Xu, Fuming Dai 한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 8 71-76 (6 pages)

Xin Liu, Rong Zeng, Shigang Gao, Lihui Xu, Fuming Dai 저
...
- 3,000원
on agarose gels. The tetra-primer ARMS-PCR systems for SdhBP225F and SdhBH272R were validated by 9 SdhB-squenced strains repeatedly. Then, sensitivity of 30 more strains were also tested by the methods, which were accordant with genotypes by sequencing and the sensitivity of conidial germination to boscalid by 100%. Thus, the methods developed in this study are proved to be rapid, inexpensive, accurate and practical for resistance detection of Botrytis cinerea caused by SdhBP225F and SdhBH272R...
726.

Genetic Variability among Different Populations of Root Knot Nematodes Based on Their Encumbrance Response to Pasteuria Isolates Using PCR-RFLP

Muhammad Kamran, Nazir Javed, Ihsan Ullah, Shahid Nazir, Shakra Jamil, Muhammad Zafar Iqbal, Huma Abbas, Sajid Aleem Khan, Muhammad Ehetisham ul Haq 한국식물병리학회 The Plant Pathology Journal 12 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 6 51-62 (12 pages)

Muhammad Kamran, Nazir Javed, Ihsan Ullah, Shahid Nazir, Shakra Jamil, Muhammad Zafar Iqbal, Huma Abbas, Sajid Aleem Khan, Muhammad Ehetisham ul Haq 저
...
- 4,100원
M. incognita with the highest frequency (52.6%) followed by M. javanica (36.8%) and M. arenaria (10.5%). The sizes of PCR products were 1.7 kb for M. incognita and M. javanica populations while populations of M. arenaria produced 1.1 kb fragment. The digestion with Hinf I yielded three different fragment length patterns on 1.5 % agarose gel. From current research it is concluded that intra-Meloidogyne genetic variability exist in RKN populations which have better encumbrance with P. penetrans.
727.

Presence of Diverse Sugarcane Bacilliform Viruses Infecting Sugarcane in China Revealed by Pairwise Sequence Comparisons and Phylogenetic Analysis

Kashif Ahmad, Sheng-Ren Sun, Jun-L? Chen, Mei-Ting Huang, Hua-Ying Ful, San-Ji Gao 한국식물병리학회 The Plant Pathology Journal 10 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 5 41-50 (10 pages)

Kashif Ahmad, Sheng-Ren Sun, Jun-L? Chen, Mei-Ting Huang, Hua-Ying Ful, San-Ji Gao 저
...
- 3,000원
Sugarcane bacilliform viruses (SCBV), which belong to the genus Badnavirus, family Caulimoviridae, are an important DNA virus complex that infects sugarcane. To explore the genetic diversity of the sugarcane-infecting badnavirus complex in China, we tested 392 sugarcane leaf samples collected from Fujian, Yunnan, and Hainan provinces for the occurrence of SCBV by polymerase chain reaction (PCR) assays using published primers SCBV-F and SCBV-R that target the reverse transcriptase/ribonuclease H...
728.

Visualization of Phytophthora palmivora Infection in Oil Palm Leaflets with Fluorescent Proteins and Cell Viability Markers

Juan C. Ochoa, Mariana Herrera, M?nica Navia, Hern?n Mauricio Romero 한국식물병리학회 The Plant Pathology Journal 13 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 3 19-31 (13 pages)

Juan C. Ochoa, Mariana Herrera, M?nica Navia, Hern?n Mauricio Romero 저
...
- 4,200원
Bud rot (BR) is the most devastating disease affecting oil palm (Elaeis guineensis) crops in Colombia. Its causal agent, Phytophthora palmivora, initiates the infection in immature oil palm leaflets producing necrotic lesions, followed by colonization of opportunistic necrotrophs, which increases disease damage. To improve the characterization of the disease, we transformed P. palmivora using Agrobacterium tumefaciens-mediated transformation (ATMT) to include the fluorescent proteins CFPSKL...
729.

Selection of Reference Genes for Real-time Quantitative PCR Normalization in the Process of Gaeumannomyces graminis var. tritici Infecting Wheat

Li-hua Xie, Xin Quan, Jie Zhang, Yan-yan Yang, Run-hong Sun, Ming-cong Xia, Bao-guo Xue, Chao Wu, Xiao-yun Han, Ya-nan Xue, Li-rong Yang 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 2 11-18 (8 pages)

Li-hua Xie, Xin Quan, Jie Zhang, Yan-yan Yang, Run-hong Sun, Ming-cong Xia, Bao-guo Xue, Chao Wu, Xiao-yun Han, Ya-nan Xue, Li-rong Yang 저
...
- 3,000원
Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression...
730.

Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification

Nam-Yeon Kim, Jong-hee Oh, Su-Heon Lee, Hong-sup Kim, Jae Sun Moon, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 5 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 12 575-579 (5 pages)

Nam-Yeon Kim, Jong-hee Oh, Su-Heon Lee, Hong-sup Kim, Jae Sun Moon, Rae-Dong Jeong 저
...
- 1,500원
Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an...

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