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71.

A comparative study of Sephadex, glass wool and Percoll separation techniques on sperm quality and IVF results for cryopreserved bovine semen

Hae-Lee Lee, Sue-Hee Kim, Dong-Beom Ji, Yong-Jun Kim* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 11 249-255 (7 pages)

Hae-Lee Lee, Sue-Hee Kim, Dong-Beom Ji, Yong-Jun Kim* 저
...
- 3,000원
The aim of this study was to compare the effects of spermatozoa separation techniques on sperm quality and in-vitro fertilization (IVF) results for cryopreserved bovine semen. Sephadex, glass wool and Percoll gradient separation techniques were used for sperm separation and sperm motility, morphology and membrane integrity were evaluated before and after separation. Also, cleavage and blastocyst developmental rate were investigated after IVF with sperm recovered by each separation technique. The...
72.

A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples

Vijayarani Kumanan, Sam R. Nugen, Antje J. Baeumner, Yung-Fu Chang* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 6 35-42 (8 pages)

Vijayarani Kumanan, Sam R. Nugen, Antje J. Baeumner, Yung-Fu Chang* 저
...
- 3,000원
simple, membrane-strip-based lateral-flow (LF) biosensor assay and a high-throughput microtiter plate assay have been combined with a reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of a small number (ten) of viable Mycobacterium (M.) avium subsp. paratuberculosis (MAP) cells in fecal samples. The assays are based on the identification of the RNA of the IS900 element of MAP. For the assay, RNA was extracted from fecal samples spiked with a known quantity of (10 to 10)...
73.

In vivo alternative testing with zebrafish in ecotoxicology

Seung-Hyeok Seok, Min-Won Baek, Hui-Young Lee, Dong-Jae Kim, Yi-Rang Na, Kyoung-Jin Noh, Sung-Hoon Park, Hyun-Kyoung Lee, Byoung 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 3 351-358 (8 pages)

Seung-Hyeok Seok, Min-Won Baek, Hui-Young Lee, Dong-Jae Kim, Yi-Rang Na, Kyoung-Jin Noh, Sung-Hoon Park, Hyun-Kyoung Lee, Byoung 저
...
- 3,000원
Although rodents have previously been used in ecotoxicological studies, they are expensive, time-consuming, and are limited by strict legal restrictions. The present study used a zebrafish (Danio rerio) model and generated data that was useful for extrapolating toxicant effects in this system to that of humans. Here we treated embryos of the naive-type as well as a transiently transfected zebrafish liver cell line carrying a plasmid (phAhRE- EGFP), for comparing toxicity levels with the...
74.

Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves

Brajesh C. Varshney, N.M. Ponnanna, Pranati A. Sarkar, Pragna Rehman, Jigar H. Shah 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 9 57-64 (8 pages)

Brajesh C. Varshney, N.M. Ponnanna, Pranati A. Sarkar, Pragna Rehman, Jigar H. Shah 저
...
- 3,000원
Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies to K99 antigen and electron microscopy. The K99 antigen from a selected field strain (SAR-14) was heat-extracted and fractionated on a Sepharose CL-4B column. Further purification was carried out by sodium deoxycholate treatment and/or ion-exchange chromatography. Monoclonal antibodies to...
75.

Testicular morphology and cauda epididymal sperm reserves of male rats exposed to Nigerian Qua Iboe Brent crude oil

I. Reginald Obidike, Igwebuike U. Maduabuchi, Shoyinka S.V. Olumuyiwa 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 1 1-5 (5 pages)

I. Reginald Obidike, Igwebuike U. Maduabuchi, Shoyinka S.V. Olumuyiwa 저
...
- 1,500원
testes of the crude oil-exposed rats was characterized by the presence of interstitial exudates, degeneration, and necrosis of spermatogenic and interstitial (Leydig) cells. Findings indicate that exposure of male rats to Nigerian Qua Iboe Brent crude oil may have adversely affected their reproductive systems. This may imply possible reproductive health hazards for animals and humans that may be exposed to this environmental pollutant, especially in areas where oil spillage is a common feature.
76.

Pharmacokinetics and bioavailability of doxycycline in ostriches (Struthio camelus) at two different dose rates

Ehab A. Abu-Basha, Nasir M. Idkaidek, Tareq M. Hantash 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 4 327-332 (6 pages)

Ehab A. Abu-Basha, Nasir M. Idkaidek, Tareq M. Hantash 저
...
- 3,000원
A bioavailability and pharmacokinetics study of doxycycline was carried out on 30 healthy ostriches after a single intravenous (IV), intramuscular (IM) and oral dose of 15 mg/kg body weight. The plasma doxycycline concentration was determined by HPLC/UV at 0 (pretreatment), 0.08, 0.25, 0.5 1, 2, 4, 6, 8, 12, 24 and 48 h after administration. The plasma concentration-time curves were examined using non-compartmental methods based on the statistical moment theory for only the higher dose. After IV...
77.

Influences of DTC and zinc supplementation on the cellular response restoration in restrained mice

Bozena Obminska-Mrukowicz, Marianna Szczypka 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 4 25-32 (8 pages)

Bozena Obminska-Mrukowicz, Marianna Szczypka 저
...
- 3,000원
The studies were conducted on Balb/c mice exposed to restraint stress twice for 12 h at 24 h intervals. Prior to restraint stress the mice were treated with sodium diethyldithiocarbamate (DTC) i.p. at a dose of 20 mg/kg five times at 48 h intervals. DTC was used per se or with zinc ions interaction, by adding zinc sulfate to drinking water at a dose of 72 μg/mouse daily. The results obtained in the study show that restraint stress causes involution of lymphatic organs, decreased the percentage...
78.

Repeated dose toxicity of alfa-cypermethrin in rats

S Manna, D Bhattacharyya, TK Mandal, S Das 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 9 241-245 (5 pages)

S Manna, D Bhattacharyya, TK Mandal, S Das 저
...
- 1,500원
The present study was performed to investigate the subacute effect of α-cypermethrin (α-CP) in rats. Alfa-cypermethrin a synthetic pyrethroid insecticide, dissolved in dimethyl sulfoxide (DMSO) and oral LD50 was investigated after administering orally different doses in rats and was determined as 145 mg/kg. Other groups of rats were given repeated daily oral dose (1/10 LD50 ) of α-CP for 30 days. The animals were sacrificed on 31st day. Activities of various enzymes, cytochrome P450 and b5...
79.

Determination of Roxithromycin by Liquid Chromatography/ Mass Spectrometry after Multiple-Dose Oral Administration in Broilers

Jong-hwan Lim, Byung-kwon Park, Hyo-in Yun 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 1호 6 35-39 (5 pages)

Jong-hwan Lim, Byung-kwon Park, Hyo-in Yun 저
...
- 1,500원
A highly sensitive and specific method for the determination of roxithromycin in broiler tissues by LC/MS was developed and validated. A dichloromethane extract of the sample was separated on C18 reversed-phase column with acetonitrile-50 mM ammonium acetate (80:20, v/v) as the mobile phase and analyzed by LC/MS via atmospheric pressure ionization/electrospray ionization interface. The limit of detection and limit of quantitation were 1 ng/g and 5 ng/g. The method has been successfully applied...
80.

Biological Rhythm in Livestock

Piccione Giuseppe and Caola Giovanni 대한수의학회 Journal of Veterinary Science 13 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 1 145-157 (13 pages)

Piccione Giuseppe and Caola Giovanni 저
...
- 4,200원
and of sensitivity and resistance to many environmental agents are often quite large and offer not only new insight into animal physiology and pathology but also diagnostic possibilities and therapeutic advantages. Chronobiology, chronophysiology and its subspecialities, like chronopharmacology and chronotherapy, will certainly play an important role in the clinical medicine of the future. Successful application of chronobiology to veterinary clinical medicine, however, depends critically on a...

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