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Downregulation of cellular prion protein inhibited the proliferation and invasion and induced apoptosis of Marek’s disease virus-transformed avian T cells
Xuerui Wan, Runxia Yang, Guilin Liu, Manling Zhu, Tianliang Zhang, Lei Liu, Run Wu* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 6 171-178 (8 pages)
Cellular prion protein (PrP) is ubiquitously expressed in the cytomembrane of a considerable number of eukaryotic cells. Although several studies have investigated the functions of PrP in cell proliferation, cell apoptosis, and tumorigenesis of mammals, the correlated functions of chicken PrP (chPrP) remain unknown. In this study, stable chPrP-downregulated Marek’s disease (MD) virus-transformed avian T cells (MSB1-SiRNA-3) were established by introducing short interfering RNA (SiRNA) targeting... -
Expression of neurotrophic factors in injured spinal cord after transplantation of human-umbilical cord blood stem cells in rats
Hyo-jin Chung, Wook-hun Chung, Jae-Hoon Lee, Dai-Jung Chung, Wo-Jong Yang, A-Jin Lee, Chi-Bong Choi, Hwa-Seok Chang, Dae-Hyun Ki 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 12 97-102 (6 pages)
We induced percutaneous spinal cord injuries (SCI) using a balloon catheter in 45 rats and transplanted human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) at the injury site. Locomotor function was significantly improved in hUCB-MSCs transplanted groups. Quantitative ELISA of extract from entire injured spinal cord showed increased expression of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3). Our results show that treatment of SCI... -
Production of α 1,3-galactosyltransferase targeted pigs using transcription activator-like effector nuclease-mediated genome editing technology
Jung-Taek Kang, Dae-Kee Kwon, A-Rum Park, Eun-Jin Lee, Yun-Jin Yun, Dal-Young Ji, Kiho Lee, Kwang-Wook Park 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 1호 11 89-96 (8 pages)
Recent developments in genome editing technology using meganucleases demonstrate an efficient method of producing gene edited pigs. In this study, we examined the effectiveness of the transcription activator-like effector nuclease (TALEN) system in generating specific mutations on the pig genome. Specific TALEN was designed to induce a double-strand break on exon 9 of the porcine α1,3-galactosyltransferase (GGTA1) gene as it is the main cause of hyperacute rejection after xenotransplantation.... -
Classical natural ovine scrapie prions detected in practical volumes of blood by lamb and transgenic mouse bioassays
Rohana P. Dassanayake, Thomas C. Truscott, Dongyue Zhuang, David A. Schneider, Sally A. Madsen-Bouterse, Alan J. Young, James B. 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 7 179-186 (8 pages)
Scrapie is diagnosed antemortem in sheep by detecting misfolded isoforms of prion protein (PrP) in lymphoid follicles of the rectal mucosa and nictitating membranes. Assay sensitivity is limited if (a) the biopsy is collected early during disease development, (b) an insufficient number of follicles is collected, or (c) peripheral accumulation of PrP is reduced or delayed. A blood test would be convenient for mass live animal scrapie testing. Currently approved techniques, however, have their own... -
Micro-computed tomography evaluation and pathological analyses of female rats with collagen-induced arthritis
Young Hee Kim, Jin Seok Kang 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 5 165-171 (7 pages)
in a time-dependent manner following collagen administration. Bone volume was significantly decreased in the tibia at weeks 3 and 4 compared to week 0 (p < 0.05 and p < 0.01, respectively). Additionally, percent bone volume was significantly reduced in the tibia at week 4 compared to week 0 (p < 0.05). In contrast, bone surface/bone volume and trabecular separation were significantly increased in the tibia of the animals at week 4 compared to week 0 (p < 0.05). Severe joint destruction with... -
Comparison of three skin-stretching devices for closing skin defects on the limbs of dogs
Vassiliki Tsioli, Lysimachos G. Papazoglou, Nikolaos Papaioannou, Dimitra Psalla, Ioannis Savvas, Leonidas Pavlidis, Ma 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 13 99-106 (8 pages)
Our objective was to evaluate the effectiveness of skin-stretching devices for closing defects on the extremities of dogs. Antebrachial skin defects were created on the limbs of 24 dogs randomly divided into three groups. Skin stretchers included staples and sutures passing through them (group A), sutures and hypodermic needles (group B), and Pavletic device (group C). Wounds on the left were further undermined in all groups. Tension and blood perfusion were assessed.... -
Eggshell apex abnormalities associated with Mycoplasma synoviae infection in layers
Eun-Ok Jeon, Jong-Nyeo Kim, Hae-Rim Lee, Bon-Sang Koo, Kyeong-Cheol Min, Moo-Sung Han, Seung-Baek Lee, Yeon-Ji Bae, Jong-Suk Mo, 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 12 579-582 (4 pages)
Eggs exhibiting eggshell apex abnormalities (EAA) were evaluated for changes in shell characteristics such as strength, thickness, and ultrastructure. Mycoplasma synoviae (MS) infection was confirmed by serological assay along with isolation of MS from the trachea and oviduct. Changes in eggshell quality were shown to be statistically significant (p < 0.01). We also identified ultrastructural changes in the mammillary knob layer by Scanning Electron Microscopy. While eggs may seem to be... -
Feasibility of utilizing the patellar ligament angle for assessing cranial cruciate ligament rupture in dogs
Jung-ha Lee, Soon-wuk Jeong 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 8 563-568 (6 pages)
The patellar ligament angle (PLA) was assessed in 105 normal stifle joints of 79 dogs and 33 stifle joints of 26 dogs with a ruptured cranial cruciate ligament (CrCL). The PLA of stifles with complete CrCL rupture was significantly lower than that of normal stifles, particularly at a flexion angle of 60∼80o in both plain and stress views. If the PLA was <90.55o on the stress view with a 60∼80o flexion angle, the dog was diagnosed with a complete rupture of the CrCL with a sensitivity of 83.9%... -
Distribution, quantitative load and characterization of Salmonella associated with swine farms in upper-northern Thailand
Pakpoom Tadee, Kittipong Kumpapong, Danai Sinthuya, Panuwat Yamsakul, Nipa Chokesajjawatee, Supachai Nuanualsuwan, Suchawan Porn 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 20 327-334 (8 pages)
This study was conducted to analyze the prevalence and quantitative loads of Salmonella spp. on pig farms in Chiang Mai, Lamphun, Thailand to assess loading levels before slaughtering. The serotype diversity, antimicrobial-resistance pattern and pulse-field type of Salmonella spp. were also characterized to assess the dynamic propagation of the pathogen. The Salmonella-positive prevalence was 246/805 (30.56%), and the quantitative loads varied from 1.48∼4.04 Log10MPN/g, with a mean ± standard... -
Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows
Hasina Santatriniaina Rasolomboahanginjatovo, Younès Chorfi*, Raynald Dupras, Louis Mills, Réjean Lefebvre 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 14 273-281 (9 pages)
The objective of this study was to determine the effects of superovulation (SOV) on serum and uterine biochemical parameters, uterine bacteriology and cytology and number of transferable embryos (TE). Dairy cows were placed on a Presynch/CIDR Synch protocol. The SOV group was superovulated, induced in estrus, and inseminated, whereas the control group was induced in estrus and inseminated without SOV. Uterine bacteriology and cytology and uterine and serum biochemical parameters were measured at...


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