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전체 선택해제
총 1,116건 검색
본문검색 결과만 보기
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Microwave Irradiation-assisted RNA Extraction from Woody Tissues for Plant Virus Detection
한국식물병리학회 The Plant Pathology Journal 3 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 14 286-288 (3 pages)
Plant tissue homogenization using a mortar or mechanical equipment has been the preferred method for obtaining high yields of total RNA; this method, however, is both time-consuming and expensive. Additionally, homogenization may generate excessive endogenous RNases, polyphenolics, and other substances that reduce the quality and quantity of RNA. In this study, we describe the microwave irradiation-assisted RNA extraction (MIRE) technique which, without tissue disruption and homogenization,... -
Species-specific Detection of Erwinia pyrifoliae by PCR Assay Using Enterobacterial Repetivive Intergenic Consensus (ERIC) Primers
한국식물병리학회 The Plant Pathology Journal 4 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 10 267-270 (4 pages)
We designed a sensitive and specific PCR-based method with enterobacterial repetitive intergenic consensus (ERIC) primer to detect Erwinia pyrifoliae, which cause shoot blight in Asian pear, from a mixed culture and infected plant materials. The primers specifically detected only E. pyrifoliae and showed no cross-reactivity with other bacterial phytopathogens. -
Spatial and Temporal Distribution of a Biocontrol Bacterium Bacillus licheniformis N1 on the Strawberry Plants
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 5 238-244 (7 pages)
Spatial and temporal distribution of Bacillus licheniformis N1 was investigated over time on the leaves, petioles and crowns of the strawberry plants. Bacterial population on the strawberry plants was quantified over time by selective plating. Bacterial population of N1 containing a plasmid pWH43G carrying green fluorescent protein (GFP) declined relatively faster on the plant surface as compared to the Strain N1 itself. However, this result was found to be enough to utilize the strain to... -
Molecular Characterization of Burkholderia cepacia Complex Isolates Causing Bacterial Fruit Rot of Apricot
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 3 223-230 (8 pages)
The Burkholderia cepacia complex isolates causing bacterial fruit rot of apricot were characterized by speciesspecific PCR tests, recA-HaeIII restriction fragment length polymorphism (RFLP) assays, rep-PCR genomic fingerprinting, recA gene sequencing, and multilocus sequence typing (MLST) analysis. Results indicated that the isolates Bca 0901 and Bca 0902 gave positive amplifications with primers specific for B. vietnamiensis while the two bacterial isolates showed different recARFLP and rep-PCR... -
Phenotypic and Genotypic Analysis of Rice Lesion Mimic Mutants
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 2호 9 159-169 (11 pages)
Lesion mimic mutant (LMM) plants display spontaneous necrotic lesions on their leaves without any pathogenic infection. Specific rice LMMs designated as spotted leaf (spl) including spl1, spl3, spl4, spl5 and spl6 are genetically known as lesion resembling disease (lrd) mutant. The inheritance patterns in the F1 and F2 progenies of these mutants are controlled by recessive genetic factors. Lesion development in the rice LMMs were controlled by both development stages and environmental factors.... -
Characteristics of Cucumber mosaic virus Infecting Zucchini in Korea
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 2호 6 139-148 (10 pages)
A virus causing stunt, yellowing, severe mosaic, malformation symptoms on leaves and uneven development and malformation on fruits of zucchini was prevalent around Goseong, Gyeongsangnam-do, Korea. A survey conducted (2004) in the Goseong area revealed about 20% virus infection rate. The disease causative identified as Cucumber mosaic virus (CMV-Z1) was further characterized. The isolate induces mosaic symptoms on Cucumis sativus, while severe mosaic, stunt and malformation on C. pepo. Thin... -
Microbial Population, Aflatoxin Contamination and Predominant Aspergillus Species in Korean Stored Rice
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 2호 4 121-129 (9 pages)
We evaluated microbial populations and aflatoxin production in unhulled and white rice from rice processing complexes of the National Agricultural Cooperative Federation in five regions in Korea and identified three predominant Aspergillus species. Fungal and bacterial populations in rice samples were significantly different between regions in 2007. Aflatoxins were also detected and varied at the levels of 2.45 - 3.43 ng per g unhulled rice grain and 1.29 - 2.09 ng per g white rice grain.... -
First Report of Cucumber mosaic virus Infecting Pinewood Coneflower (Rudbeckia bicolor) in Korea
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 14 93-98 (6 pages)
A virus isolate causing symptoms of yellow mosaic, fern leaves, malformation and plant necrosis on Rudbeckia bicolor was prevalent around Pyeongchang area in Korea. The causal virus was identified as Cucumber mosaic virus (CMV) using characteristics from biological, serological and molecular analyses and named as CMV-Rb. CMV-Rb caused mosaic on Nicotiana benthamiana, N. tabacum, Capsicum annuum, and Lycopersicon esculentum. However, typical local lesions did not develop on inoculated Pisum... -
First Report of Black Spot Caused by Alternaria alternata on Grafted Cactus
한국식물병리학회 The Plant Pathology Journal 3 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 11 80-82 (3 pages)
A stem spot disease was observed on Gymnocalycium mihanovichii (Korean name: Bimoran), a scion of graftcactus, in major growing areas of Goyang and Eumseong, Korea during 2008 and 2009. Typical symptoms were initial blackish brown lesions produced mainly on areoles and scars of injured stem tissues, gradually becoming large black spots. A causal organism isolate CD2-7A isolated from the infected stems was identified as Alternaria alternata based on its morphological characteristics and confirmed... -
Screening of Rice Blast Resistance Genes from Aromatic Rice Germplasms with SNP Markers
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 10 70-79 (10 pages)
Rice blast is one of the serious devastating diseases. This study was carried out to determine the genetic diversities of blast resistance (R) genes form 86 accessions of aromatic rice with eight SNP markers, z4792, zt4792, z60510, zt6057, k6415, k6411, k39575 and t256, which showed the close-set linkage to 6 major genes, Piz, Piz-t, Pik, Pik-m, Pik-p, and Pit. Four accessions of indica type, Mayataung, Yekywin Yinkya Hmwe, Basmati9-93, and Basmati5854, showed the positive amplicons of six major...
