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Isolation and characterization of equine amniotic membrane-derived mesenchymal stem cells
Min-Soo Seo, Sang-Bum Park, Hyung-Sik Kim, Jun-gu Kang, Joon-Seok Chae*, Kyung-Sun Kang* 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 6 151-159 (9 pages)
Recent studies have shown that mesenchymal stem cells (MSCs) are able to differentiate into multi-lineage cells such as adipocytes, chondroblasts, and osteoblasts. Amniotic membrane from whole placenta is a good source of stem cells in humans. This membrane can potentially be used for wound healing and corneal surface reconstruction. Moreover, it can be easily obtained after delivery and is usually discarded as classified waste. In the present study, we successfully isolated and characterized... -
Development of a monoclonal antibody against deoxynivalenol for magnetic nanoparticle-based extraction and an enzyme-linked immunosorbent assay
Hyuk-Mi Lee, Sung-Ok Song, Sang-Ho Cha, Sung-Bok Wee, Karyn Bischoff, Sung-Won Park, Seong-Wan Son, Hwan-Goo Kang*, Myung-Haing 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 5 143-150 (8 pages)
Monoclonal antibody (mAb, NVRQS-DON) against deoxynivalenol (DON) was prepared. DON-Ag coated enzyme linked immunosorbent assay (ELISA) and DON-Ab coated ELISA were prepared by coating the DON-BSA and DON mAb. Quantitative DON calculation ranged from 50 to 4,000 ng/mL for DON-Ab coated ELISA and from 25 to 500 ng/mL for DON-Ag coated ELISA. 50% of inhibitory concentration values of DON, HT-2, 15-acetyl-DON, and nivalenol were 23.44, 22,545, 5,518 and 5,976 ng/mL based on the DON-Ab coated ELISA.... -
Antiviral effect of dietary germanium biotite supplementation in pigs experimentally infected with porcine reproductive and respiratory syndrome virus
Bock-Gie Jung, Jin-A Lee, Bong-Joo Lee* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 4 135-141 (7 pages)
Germanium biotite (GB) is an aluminosilicate mineral containing 36 ppm germanium. The present study was conducted to better understand the effects of GB on immune responses in a mouse model, and to demonstrate the clearance effects of this mineral against Porcine reproductive and respiratory syndrome virus (PRRSV) in experimentally infected pigs as an initial step towards the development of a feed supplement that would promote immune activity and help prevent diseases. In the mouse model,... -
In vitro effects of meloxicam on the number, Foxp3 expression, production of selected cytokines, and apoptosis of bovine CD25+CD4+ and CD25−CD4+ cells
Tomasz Maślanka*, Jerzy Jan Jaroszewski 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 3 125-134 (10 pages)
The purpose of this study was to evaluate the effect of meloxicam (MEL) on selected immune parameters of bovine CD25CD4+, CD25CD4+, and CD25−CD4+ cells. Peripheral blood mononuclear cells (PBMCs) collected from 12-month-old heifers were treated with MEL at a concentration corresponding to the serum level of this medication following administration at the recommended dose (MEL 5 × 10 M) and at a concentration 10 times lower (MEL 5 × 10 M). After 12 and 24 h of incubation with the drug,... -
Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea
Eun-Jin Choi, Chang-Hee Lee, Jae-Young Song, Hee-Jong Song, Choi-Kyu Park, Byounghan Kim, Yeun-Kyung Shin* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 2 115-124 (10 pages)
The high genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) has been an obstacle to developing an effective vaccine for porcine reproductive and respiratory syndrome (PRRS). This study was performed to assess the degree of genetic diversity among PRRSVs from Korean pig farms where wasting and respiratory syndrome was observed from 2005 to 2009. Samples from 786 farms were tested for the presence of PRRSV using reverse transcription PCR protocol. A total of 117 farms... -
Sporozoite proteome analysis of Cryptosporidium parvum by one-dimensional SDS-PAGE and liquid chromatography tandem mass spectrometry
AMAM Zonaed Siddiki* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 1 107-114 (8 pages)
Despite the development of new technologies, new challenges still remain for large scale proteomic profiling when dealing with complex biological mixtures. Fractionation prior to liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis is usually the preferred method to reduce the complexity of any biological sample. In this study, a gel LC-MS/MS approach was used to explore the stage specific proteome of Cryptosporidium (C.) parvum. To accomplish this, the sporozoite protein of C.... -
Emergency euthanasia of cattle challenged with Escherichia coli O157:H7 - A case study for evaluating the response to an infectious disease outbreak
Brandon H. Gilroyed, Tim Reuter, John P. Kastelic, Tim A. McAllister* 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 16 103-106 (4 pages)
In the event of an infectious disease outbreak in cattle, carcasses must be disposed of in a rapid and contained manner. This brief communication details injection of a barbiturate to euthanize cattle inoculated with Escherichia coli O157:H7 followed by carcass composting in a manner that prevents the spread of infectious agents. -
Genetic IS901 RFLP diversity among Mycobacterium avium subsp. avium isolates from four pheasant flocks
Monika Moravkova, Jiri Lamka, Michal Slany, Ivo Pavlik* 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 15 99-102 (4 pages)
IS901 RFLP analysis of 36 Mycobacterium avium subsp. avium (MAA) isolates from 15 pheasants (Phasianus colchicus) and two goshawks (Accipiter gentilis) from four pheasant farms was performed. Using this method, six different IS901 RFLP types (E, F, G, M, Q, and V) were identified. The distribution of IS901 RFLP profiles was tightly linked to individual flocks. Matching IS901 RFLP profiles observed in the present study indicate MAA transmission between pheasants and goshawks in the same locality.... -
Serotype- and serogroup-specific detection of African horsesickness virus using phage displayed chicken scFvs for indirect double antibody sandwich ELISAs
Wouter van Wyngaardt*, Cordelia Mashau, Isabel Wright, Jeanni Fehrsen 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 14 95-98 (4 pages)
There is an ongoing need for standardized, easily renewable immunoreagents for detecting African horsesickness virus (AHSV). Two phage displayed single-chain variable fragment (scFv) antibodies, selected from a semi-synthetic chicken antibody library, were used to develop double antibody sandwich enzyme-linked immunosorbent assays (DAS-ELISAs) to detect AHSV. In the DAS-ELISAs, the scFv previously selected with directly immobilized AHSV-3 functioned as a serotype-specific reagent that recognized... -
Abundance of biting midge species (Diptera: Ceratopogonidae, Culicoides spp.) on cattle farms in Korea
Jae-Ku Oem, Joon-Yee Chung, Mee-Soon Kwon, Toh-Kyung Kim, Tae-Uk Lee, You-Chan Bae* 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 13 91-94 (4 pages)
Culicoides biting midges were collected on three cattle farms weekly using light traps overnight from May to October between 2010 and 2011 in the southern part of Korea. The seasonal and geographical abundance of Culicodes spp. were measured. A total of 16,538 biting midges were collected from 2010 to 2011, including seven species of Culicoides, four of which represented 98.42% of the collected specimens. These four species were Culicodes (C.) punctatus (n = 14,413), C. arakawae (n = 1,120), C....


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