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전체 선택해제
총 915건 검색
본문검색 결과만 보기
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A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis
Miguel Salgado*, Cristobal Verdugo, Cord Heuer, Pedro Castillo, Patricia Zamorano 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 9 233-239 (7 pages)
The final PCR results for 11 samples analyzed with the QIAamp and UACH methods or ones examined with the QIAamp and CTAB methods were in agreement. Complete (100%) agreement was observed between data from the CTAB and UACH methods. CP values for the UACH and CTAB techniques were not significantly different, while the UACH method yielded significantly lower CP values compared to the QIAamp kit. The proposed extraction method combines reliability and efficiency with simplicity and lower cost. -
Evaluation of PCR inhibitory effect of enrichment broths and comparison of DNA extraction methods for detection of Salmonella Enteritidis using real-time PCR assay
Ji-Yeon Hyeon, In-Gyun Hwang, Hyo-Sun Kwak, Chankyu Park, In-Soo Choi, Kun-Ho Seo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 8 143-149 (7 pages)
The best enrichment broth and DNA extraction scheme was determined for rapid and sensitive detection of Salmonella Enteritidis in steamed pork using real-time PCR. The inhibitory effect of commonly used Salmonella enrichment broths, Rappaport-Vassiliadis (RV) and Muller-Kauffmann tetrathionate with novobiocin (MKTTn), on real-time PCR was confirmed. The inhibition of PCR was statistically significant (p ＜ 0.05) in RV and MKTTn, as compared with buffered peptone water (BPW) or phosphate-buffered... -
Short Communication : Effective DNA extraction method to improve detection of Mycobacterium avium subsp. paratuberculosis in bovine feces
Hong Tae Park , Min Kyoung Shin , Kyung Yong Sung , Hyun Eui Park , Yong Il Cho , Han Sang Yoo 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 3 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2014, 제 54권 제 1호 10 55-57 (3 pages)
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Simple and Reliable DNA Extraction Method for the Dark Pigmented Fungus, Cercospora sojina
한국식물병리학회 The Plant Pathology Journal 4 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 15 289-292 (4 pages)
the plant pathogenic fungus Cercospora sojina. Total DNA yield obtained by this method was approximately 1 mg/g of mycelia (fresh weight), and the mean ratio of A260/A280 and A260/A230 were 2.04 and 2.1, respectively. The results of random amplified polymorphic DNA (RAPD) analysis, digestion with restriction enzymes, and Southern hybridization indicated that polysaccharides were effectively removed by this method, and the resulting DNA was sufficient for use in subsequent molecular analysis. -
A Quick and Safe Method for Fungal DNA Extraction
한국식물병리학회 The Plant Pathology Journal 4 Pages
한국식물병리학회 The Plant Pathology Journal 2009, 25권 1호 17 108-111 (4 pages)
including cloning and genotyping, have become routine in fungal research laboratories. However, preparation of high-quality DNA from fungal tissue requires much time and labor and is often a limiting step for high-throughput experiments. We have developed a quick and safe (QS) DNA extraction method for fungi. Time efficiency and safety in the QS method were achieved by using plate-grown mycelia as the starting material, by eliminating phenol-chloroform extraction procedures, and by deploying a... -
A Simple Method for Extraction of High Molecular Weight DNA fromPorphyra Tenera (Rhodophyta) Using Diatomaceous Earth
김태훈, 황미숙, 송주동, 오민혁, 문용환, 정익교, 류태형, 이춘환, Kim. Tae-Hoon, Hwang. Mi-Sook, Song. Ju-Dong, Oh. Min-Hyuk, Moon. Yong-Hwan, Chung. Ik-Kyo, Rhew. Tae- 한국조류학회(藻類) Algae 6 Pages
한국조류학회(藻類) Algae 2006, Vol.21 No.2 261-266 (6 pages)
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A Simple Method for DNA Extraction from Red Algae
이유경, 이홍금, Lee. Yu Gyeong, Lee. Hong Geum 한국조류학회(藻類) Algae 5 Pages
한국조류학회(藻類) Algae 2003, Vol.18 No.1 65-69 (5 pages)
