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A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis
Miguel Salgado*, Cristobal Verdugo, Cord Heuer, Pedro Castillo, Patricia Zamorano 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 9 233-239 (7 pages)
PCR is a highly accurate technique for confirming the presence of Mycobacterium avium subsp. paratuberculosis (Map) in broth culture. In this study, a simple, efficient, and low-cost method of harvesting DNA from Map cultured in liquid medium was developed. The proposed protocol (Universidad Austral de Chile [UACH]) was evaluated by comparing its performance to that of two traditional techniques (a QIAamp DNA Stool Mini Kit and cethyltrimethylammonium bromide [CTAB] method). The results were... -
Studies on mastitis, milk quality and health risks associated with consumption of milk from pastoral herds in Dodoma and Morogoro regions, Tanzania
Esron D. Karimuribo, Lughano J. Kusiluka, Robinson H. Mdegela, Angolwisye M. Kapaga, Calvin Sindato, Dominic M. Kambarage 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 7 213-221 (9 pages)
The prevalence of mastitis, milk quality and health risks associated with milk consumption were investigated on 96 randomly selected traditional herds in Dodoma rural and Mvomero districts of Tanzania. Mastitis was investigated based on clinical signs, microbiology and California mastitis test (CMT), while milk quality was evaluated using total viable count (TVC)and total coliform count (TCC). Animals were tested for tuberculosis using a single comparative intradermal tuberculin test. The... -
Preparation of Nanomaterial Wettable Powder Formulations of Antagonistic Bacteria from Phellodendron chinense and the Biological Control of Brown Leaf Spot Disease
Yanling Zeng, Han Liu, Tianhui Zhu, Shan Han, Shujiang Li 한국식물병리학회 The Plant Pathology Journal 17 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 2 215-231 (17 pages)
and ultraviolet resistance by 16%, 12%, and 38.9%, respectively, and nano-SiO2 was added to the fermentation process. The best formula for the wettable powder was 35% kaolin, 4% polyethylene glycol, 8% Tween, and 2% humic acid. The following quality test results for the wettable powder were obtained: wetting time 87.0 s, suspension rate 80.33%, frequency of microbial contamination 0.08%, pH 7.2, fineness 95.8%, drying loss 1.47%, and storage stability ≥83.5%. A pot experiment revealed that the... -
Biological Control of Gom-chwi (Ligularia fischeri) Phytophthora Root Rot with Enterobacter asburiae ObRS-5 to Suppress Zoosporangia Formation and Zoospores Germination
Dayeon Kim, Sang Yeob Lee, Seong Ho Ahn, Ji Hee Han, Jin Woo Park 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 3호 5 244-254 (11 pages)
by 87.4% and 66.7%, respectively. In addition, culture filtrate of E. asburiae ObRS-5 also significantly inhibited sporangium formation and zoospore germination by 97.0% and 67.6%, respectively. Soil drenched bacte- rial cells, filtrate, and culture solution of E. asburiae ObRS-5 effectively suppressed Phytophthora root rot by 63.2%, 57.9%, and 81.1%, respectively. Thus, E.asburiae ObRS-5 could be used as a potential agent for the biological control of Phytophthora root rot infecting gom-chwi. -
Genetic Variation and Biological Control of Fusarium graminearum Isolated from Wheat in Assiut-Egypt
한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 2호 8 145-156 (12 pages)
extensive yield and quality loss of wheat and other cereal crops. Twelve isolates of F. graminearum were collected from naturally infected spikes of wheat from Assiut Egypt. These isolates were compared using SRAP. The results indicated distinct genetic groups exist within F. graminearum, and demonstrated that these groups have different biological properties, especially with respect to their pathogenicity on wheat. There were biologically significant differences between the groups; with group... -
Research Notes : Reverse Transcription Polymerase Chain Reaction-based System for Simultaneous Detection of Multiple Lily-infecting Viruses
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 3호 13 338-343 (6 pages)
lily mottle virus (LMoV), lily symptomless virus (LSV). Leaf samples were collected at lily-cultivation facilities located in the Kangwon province of Korea and used to evaluate the detection system. Simplex and multiplex RT-PCR were performed using virus-specific primers to detect singleor mixed viral infections in lily plants. Our results demonstrate the selective detection of 3 different viruses (CMV, LMoV and LSV) by using specific primers as well as the potential of simultaneously detecting... -
Graphene: an emerging material for biological tissue engineering
SangKyu Lee, Hyun Kim, BongSup Shim 한국탄소학회 Carbon Letters 13 Pages
한국탄소학회 Carbon Letters 2013, Vol.14 No.2 1 63-75 (13 pages)
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Lifecycle cost assessment of best management practices for diffuse pollution control in Han River Basin
Soyoung Lee, Marla C. Maniquiz-Redillas, Jeong Yong Lee, Hyunsaing Mun, Lee-Hyung Kim 한국습지학회 한국습지학회지 8 Pages
한국습지학회 한국습지학회지 2016, Vol.18 No.4 13 448-455 (8 pages)
pond to 8,888 $/yr infiltration system. The highest LCC were observed in constructed wetland ($418,324) while vegetated system had the lowest LCC ($210,418). LCC of BMPs was not so high as compared with the conventional treatment facility and sewage treatment plant. On the other hand, the relationship of removal efficiency on unit cost for TSS and TN was significant. This study will be used to design the cost effective BMP for diffuse pollution management and become models for LCC analysis. -
Analysis of Water Quality improvement of Upper Stream for the Chung-ju Dam according to Reducing Pollution Using QUAL2E Model
Chang-Wha Han, Jong-Seok Park, Jun-Shik Shin, Jae-Joo Lee, Ui-Gyu Hwang, Kyoung-Hoon Rhee 한국습지학회 한국습지학회지 10 Pages
한국습지학회 한국습지학회지 2014, Vol.16 No.1 1 1-10 (10 pages)
The research grasps water quality contamination source by investigating the data that are superordinate laws and the related plans implemented for establishing water quality improvement measures of upper stream for Chung-Ju Dam, present conditions of the hydraulic and hydrology, the present conditions and plans of environment basic facilities, and present conditions of main contamination source’s occurrence load and discharge load, etc. The research used the QUAL2E simulation which is being...


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