This experiment was carried out to investigate the physiological characteristics of isolated bacteria, Ferrobacillus ferooxidans from copper mine water in Korea. The results obtained were as follows ; 1. The optimum pH range for the growth of these bacteria was 2.0-3.0 and optimum temperature was $20^{circ}C$-$30^{circ}C$. 2. The oxidation curves of ferrous iron to the ferric iron ran parallel with the growth curves. 3. The optimum nitrogen concentration was 400-800 ppm and the minimal flow rate of air for the maximal growth of the bactria was 70 ml air/min./200ml medium. 4. The growth of these bacteria was inhibited by the absence of ferrous iron and by the addition of sulfur. 5. Ferrous iron at a concentration of 9000 ppm, appeared to be optimum for the most rapid growth of Ferrobacillus ferrooxidans.