A strain of Streptomyces sp producing chitinase was isolated from soil and its cultural condition and some properties of this enzyme were investigated. When 0.375 per cent of glucose was added to basal medium, this organism produced the most quantities of this enzyme after shaking culture at 3$0^{circ}C$ for 48 hrs., while the production of the enzyme was repressed at the more concentration of glucose than that. The enzyme had a optimal pH of 7.0, optimal temperature of 5$0^{circ}C$ and the activity of that was not decreased by heat treatment for 20 minute at 7$0^{circ}C$. And then the activity was increased by Co$^{2+}$ but was slightly inhibited by Hg$^{2+}$, Ni$^{2+}$, Pb$^{2+}$.EX> 2+/.