전분질로부터 효모균체를 직접 생산하기 위하여 분리 동정된 전분이용성 효모, Sp. holsaticus FRI Y-5의 기본적인 배양조건에 대하여 검토하였다. 이 균주는 산소를 절대적으로 요구하며, 배지의 초기 pH가 6.9일 때 최대균체량 7.2 g/l, 균체수율 0.27, 비성장속도 $0.14hr^{-1}$로 가장 좋은 생육현상을 보여 주었고 배양온도는 $23^{circ}C$가 적온이었다. 균체생육의 활성화 에너지 Ea는 17.33 kcal/mole로 산출되었다. 검토된 13종의 질소원 가운데 $(NH_2)_2CO$가 3일 배양후 균체량이 10.69 g/l로 가장 많았으며 이때 균체수율은 0.451이었다. $SO_4;^{-2}$염으로서 $Mg^{+2},;K^+$ 및 $Zn^{+2}$와 유기영양원으로서 yeast extract도 균체증식에 좋은 영향을 주는 것으로 판명되었다.
It was investigated under several cultural conditions to produce biomass directly from starch by an strictly aerobic and amylolytic yeast, Sporobolomyces holsaticus FRI Y-5. Its optimal temperature and initial pH of medium for growth were $23^{circ}C$ and 6.9, respectively. Activation energy, Ea, for growth was calculated to be 17.33 Kcal/mole from the Arrhenius relationship. When each of 13 nitrogen sources was added to the basal medium, $(NH_2)_2CO$ had the best effect, on which concentration of cell after 3 day incubation was 10.6 g/l and cell yield was 0.451. The yeast growth was affected by $MgSO_4,;K_2SO_4;and;ZnSO_4$ as a mineral source and was best on the medium containing all of them. The addition of yeast extract (5g/l) could enhance the production of biomass and cell yield to 77% and 32%, respectively.
