Holstein cows being raised in the Kangweon district were examined on the values of blood chemistry in infertile cows. The results obtained were as follows; 1. Total serum protein was 7.52$pm$0.45g/100ml 2. Serum albumin was 3.06$pm$0.28g/100ml 3. In values of serum globulin, $alpha$1-globulin, $alpha$2- globulin, $eta$-globulin and ${gamma}$-globulin were 0.79$pm$0.14, 0.60$pm$0.10, 0.86$pm$0.13 and 2.21$pm$0.35ng/100ml, respectively. 4. A/G ration was 0.69$pm$0.09 5. Total cholesterol in serum was 119.73$pm$34.52mg/100ml 6. Blood glucose was 55.24$pm$6.46mg/100ml 7. Serum calcium was 9.27$pm$0.87mg/100ml 8. Serum inorganic phosphorus was 5.74$pm$0.86mg/100ml 9. Serum glutamic oxalacetic transaminase was 47.90$pm$11.02I$mu$/l 10. Serum glutamic pyruvic transaminase was 14.20$pm$3.02I$mu$/l