This study was carried out to elucidate the effect of different time and severity of defoliation on the dry matter accumulation in grain of rye (cv, Paldanghomil) and triticale (cv, Sinkihomil). The weight of the 2nd leaf blade was the greatest and this was followed by the 3rd and flag leaf in that order. The chlorophyll content in leaf blade and sheath were decreased at lower leaf position (flag > 2nd > 3rd). The average chlorophyll content of rye was higher than that of triticale. The earlier and severer defoliation was made, the more grain weight was its was decreased. As the time of defoliation was made later and the degree of its was small, 1,000 grain weight was increased. The results showed that the effect of defoliation on 1,000 grain weight followed the order of the flag, the 2nd and the 3rd leaf. The greatest 1,000 grain weight was observed at the middle part of panicle, which followed by the top and bottom part in that order.