The objectives of this study were investigation of the sutiable propagation method by softwood cuttings of female Ilex serrata, the comparison of sex determination of Ilex serrata after seeding and the fruiting percentage of the Ilex serrata male and female. The results obtained were as follows. 85 percentage of the seed were germinated with three months cold stratification. 35 percentage of female plant were observed after seeding. Cuttings should be rooted on late June using a quick dipping of 1,000ppm IBA solution. The rooting rate of the softwood cuttings treated IBA 1,000pm on June 22 in a closed moist chamber with 50% shade screen was 72.2 percentage. The suitable planting of male and female of Ilex serrata was male one versus female thirteen in a distance of 50 meter.