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  • 정자처리와 공배양이 체외성숙된 돼지 난포란의 분할에 미치는 영향
  • Effect of Sperm Treatment and Co-culture on cleavage of Porcine Oocytes Matured In Vitro
저자명
이장희,김창근,정영채,박충생
간행물명
韓國受精卵移植學會誌
권/호정보
1994년|9권 3호|pp.269-277 (9 pages)
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한국수정란이식학회
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

The objective of this study was to develop an effective in vitro production system capable of obtaining more porcine embryos from immature oocytes These experiments were conducted to examine the effect of sperm factor on the IVF and IVD, and the effect of coculture with somatic cells on the IVD of embryos. Although the concentration of epididymal sperm for IVF did not affect on cleavage rate, but 5 x 105 sperm/mi showed the highest cleavage rate(48.7%) and the developmental potential of IVF oocytes from this concentration was also greatly higher (P<o.05) than those from other sperm. concentrations. The treatment with heparin for sperm capacitation significantly(P<0.05) increased the cleavage rate of oocytes whereas the cleavage index between the oocytes fertilized with caffeine- or heparin-treated sperm did not differ but these chemical sperm treatments greatly increased the index compared with the control. The highest cleavage rate(47.7%) was obtained by IVF with 4$^{circ}C$-stored sperm for l2hrs and the cleavage rate from fresh sperm was significantly higher (P<0.05) than that from frozen sperm, but the developmental potential after IVF was slightly high from the frozen sperm. The cleavage rate of IVF oocytes cocultured with oviductal epithelial cells and cumulus cells was 76.3% and 72.9%, respectively. There was no difference between two coculture systems but this rate was significantly higher(P<0.05) than that of medium alone(42.0%).