In vitro fertilization(IVF) derived morula and blastocyst embryos were bisected by a simple method and cultured in vitro without zona pellucida And also bisected embryos were frozen-thawed and cultured in vitro) to evaluate the survival rate. The results obtained were as follows : The average number of grade I or II immature follicular oocytes recovered by slicing method per ovary was 11.9 from 142 ovaries. Following in vitro fertilization, the rates of cleavage and in vitro development to morula and blatocyst were 61.7 and 32.2% respectively. The successful bisection rate of IVE embryos was 67.51%, and the embryos of blastocyst stage were bisected successfully at significantly(P<O.O5) higher rate, compared with the morula stage embryos. The survival rate of bisected embryos following 24 hours culture was 32.19%, and significantly(P<9.05) higher in blastocyst stage than morula stage. The survival rate of bisected-frozen IVF embryos following post-thaw culture for 24 hours was 16.62%, being no significant difference between embryo stages at bisection: