This experiment was carried out to evaluated biological availability of calcium inthe sea mustard, sea tangle and sea lettuce in the rats. Standard diets were made to supply 0.3% of Ca from CaCO3. Consequently, levels each test ingredients to be added were sets by their Cacontents. The ingredients such as sea mustard, sea tangle and sea lettuce wereadded to basal diet at levels of 15.23%, 12.66% and 23.42% respectively. Ingredients of the basal diet were soybean protein, corn oil, alpha-cellulose, methionine, choline cholride and vitamin and mineral premix. Dextrose was added at level up to 100%. Amount of soybean protein was adjustable to make all the diets isontrogenous. Rats were fed ad libitum the diets for 13 days. The results were summarized as follow: 1. Serum calcium levels of the sea mustard group(15.23%) were higher than those of the other group, but rats fed sea lettuce diets appeared to have the lowest value. 2. Ca concentration in tibia were lower in rats fed with sea lettuce, rather than in those with standard diet. 3. Ca concentration in femur of the rats fed seaweeds did not show any significant difference among groups. 4. In the liver weight in groups fed on the all experimental groups were decreased more than that in the case of the standard diet groups.