Experimental studies were carried out on the use of supercritical fluid in enzymatic hydrolysis of cellulose. In order to effectively perform the hydrolysis the enzyme has to maintain stability and activity in the supercritical carbon dioxide solvent. In the experiment it was found that the stability of cellulase was maintained up to 160 atm for 90 min at $50^{circ}C$. In the enzymatic hydrolysis of cellulose at supercritical conditions using carbon dioxide at 80 atm and $50^{circ}C$ for 90 min, the results showed that glucose yield was 100%, which was 1.5 times as compared to that in atmospheric condition when the substrate (Avicel) concentration was 20 g/L. For the substrate concentration of 60 g/L, the glucose yield was increased by 1.2 times as compared to that in atmospheric condition.