By inserting Boa-tang into culture median of lymphocytes from spleen of rat and lymphocytes in human peripheral blood The results were as follow. 1. In case of spleen lymphocytes of rat, cultures of lymphocytes did not significantly increased which were inserted Boa-tang $100{mu}g/ml$, and which were inserted $10{mu}g/ml$ at 3rd day 2. In case of spleen lymphocytes of rat, cultures of lymphocytes were significantly increased which were inserted Boa-tang $10{mu}g/ml$ with Con A $2.5{mu}g/ml$ at 2nd, 3rd day, and Boa-tang $1{mu}g/ml$ with ConA $2.5{mu}g/ml$ at 3rd day than inserted ConA $2.5{mu}g/ml$ 3. In case of lymphocytes in human peripheral blood, cultures of lymphocytes were significantly increased which were inserted Boa-tang $100{mu}g/ml$ and $10{mu}g/ml$ at 2nd day to 5st day, and which were inserted Boa-tang $1000{mu}g/ml$ at 2nd day to 5th day, and which were inserted $1{mu}g/ml$ at 2nd day By looking at the following results, in case of rat cultures of lymphocytes were significantly increased which were inserted the lower density, but in case of human cultures of lymphocytes were significantly increased which were inserted the higher density.