In rehabilitation programs involving muscle re-education and endurance exercise, it is necessary to confirm when fatigue occurs. It is also necessary to quantify fatigue, to confirm whether the muscle has been exercised sufficiently. In general, as fatigue occurs, the force-generating ability of the muscle is reduced. If the median frequency (MDF) obtained from electromyogram (EMG) power spectrum is correlated highly with work, then the timing and degree of fatigue may be confirmed. This study examined the relationship between work and MDF obtained from the EMG power spectrum during repetitive isokinetic exercise. Surface EMG signals were collected from biceps brachii and vastus lateralis of 52 normal subjects (26 males, 26 females) at $120^{circ}/sec$ and $60^{circ}/sec$ while performing an isokinetic exercise. The exercise was finished at 25% of peak work. MDF data was obtained using a moving fast Fourier transformation (FFT), and random noise was removed using the inverse FFT, then a new MDF data was obtained from the main signal. There was a high correlation between work and MDF during repetitiv isokinetic exercise in the biceps brachii and vastus lateralis of males and the biceps brachii of females (r=.50~.77). However, there was a low correlation between work and MDF in the vastus lateralis of females (r=.06~.19).