Recently, As the higher frequency in clock of digital system being demanded and the density of circuits gets high for purpose of making light and minimizing system, the study for solution of digital signal distortion being interested. In this paper, the distortion of square pulse caused by dispersion as it propagates along a single microstrip line and crosstalk between lines on the Multi-Transmission Lines (MTL) is investigated. The dispersion and crosstalk of pulse signals is analyzed regarding to the structure of transmission line such as relative permittivity, substrate height, strip width of the microstrip line and pulse width of signal pulse.