The aim of study was to elucidate the antibiosis of the Sophora Radix extracts by ethanol solvent on bacterias. The antibiosis on bacterias was examined in moisture water included the Sophora Radix extract. The results are as follows; Minimum inhibitory concentration (MIC) of Sopora Radix extracts showed markedly lower on gram positive bacterias($0.0125{sim}0.025$) such as aureus, B. subtilis, S. epidermids than that on gram negative bacterias such as E. coli, P. aeruginosa(>10). The MIC of moisture water included the Sophora Radiix which extracted by ethanol solvent was very low which meaned good antibiosis($0.0063{sim}0.5$). The antibiosis of moisture water included the Sophora Radiix was improved by adding the ethanol on some baterias. In conclusion, Sophora Radix had excellent antibacterial effect. Therefore, it is expected that Sophora Radix can be practically used as the natural material of functional cosmetics for preventing the Bacterial inhibitory in skin.