Sedimentation basin plays an important role in urban water treatment, and there are many complicated phenomena which need to be understood for efficient design and control of it. Especially, the study on the improvement of settling efficiency is required. In this study, commercial CFD (Computational Fluid Dynamics) program, FLUENT, and particle tracking method were used to simulate the flow in sedimentation basin, and to predict the settling efficiency. Computational domain of real scale was made, and detail factors such as porous wall, and outlet trough were considered instead of being simplified. The simulation results were compared with the experimental data to calibrate the parameters of particle tracking method. Sensitivity analysis showed that the particle diameter had more significant effects on settling efficiency than the particle density. The computation results gave the best agreements with the experimental data, when the value of particle diameter was 26.5 ${mu}{ extrm}{m}$.