This experiment was undertaken to investigate the atomization characteristics of the low viscosity biodiesel blended fuel and ultrasonic energy added one. Test fuels were conventional diesel fuel and biodiesel fuel. We compared to the characteristics of viscosity and surface tension, SMD between low viscosity biodiesel blended fuel and ultrasonic energy added one. Sauter mean diameter was measured under the variation of the spray distance. Viscosity and surface tension was measured under the variation of the time trace. To measure the droplet size, we used the Malvern system 2600C. Droplet size distribution was analyzed from the result data of Malvern system. Through this experiment, we found that the condition of the ultrasonic energy added situation had smaller Sauter mean diameter of droplet, viscosity and surface tension than that of the conventional situation.