Purpose: The Purpose of this study was to identify the relationship between 7S factors of the nursing organizational culture and organizational effectiveness. Method: The data were gathered from the self-reported questionnaires of 717 nurses who work for eight different general hospitals located around Seoul and Kyounggi province. The period of data collection was from November 12 to December 7, 2002. For data analysis, descriptive statistics, clustering analysis, and t-test with SPSS Program were used. Result: The nurses who highly perceived 7S factors of nursing organizational culture showed higher job satisfaction and organizational commitment in comparison with the nurses who lowly perceived 7S factors of nursing organizational culture. And the nurses who highly perceived 7S factors of nursing organizational culture showed higher organizational citizenship behavior in comparison with the nurses who lowly perceived 7S factors of nursing organizational culture. Among subdimension of organizational citizenship behavior, altruism and civic virtue were significant. Conclusion: From the above results, the high group with 7S factors of nursing organizational culture has strong culture, therefore nursing organization with strong culture is very implicative to enhance the organizational effectiveness.