A high-speed spindle can be very sensitive to rotating mass unbalance which has harmful effect on many machine tools. Therefore, the balancing procedure to reducevibration in rotating system is certainly needed for all high-speed spindles. An active balancing program using influence coefficient method and an active balancing device of an electro-magnetic type have been applied to the developed high-speed spindle system in this study. A reliable gain-scheduling control using influence coefficients of the reference model although system characteristics are changed is applied. The stability of reference influence coefficients is verified by frequency response functions. The active balancing experiment for the developed high-speed spindle during operation is well performed with an active balancing program and device. As a result, controlled unbalance responses are below the vibration limit at all rotating speed ranges with critical speed.