This study analyzed the contents of vitamin C, polyphenol compounds, and evaluated the inhibitory activities against xanthine oxidase and tyrosinase of Lepista nuda extract prepared by the reflux and microwave-assisted methods. The vitamin C content of the fresh L. nude was $9.92{pm}0.02$ mg/100 g. The content of phenolic compounds in the fresh L. nuda was $1.87{pm}0.02;mg/g$. The ethanol extracts prepared by reflux method and microwave-assisted method showed the highest phenol contents of $12.06{pm}0.12;mg/g;and;11.84{pm}0.17;mg/g$, respectively. The inhibitory rates on xanthine oxidase and tyrosinase of ethanol extracts prepared by the microwave assisted method showed the highest value of 99.78% and 30.30% at the concentration of $3,000{mu}g/mL$. The inhibitory activities on xanthine oxidase and tyrosinase were increased depending on the extract concentration.