A new species, Pratylenchus gongjuensis n. sp. was isolated from chrysanthemum roots at Gongju, Chungnam Province, Korea. Morphological characteristics of P. gonguensis closely resemble to those of P. subpenetrans, but body length is much longer (female; $518-626{mu}m$ vs. $330-481{mu}m$, male; $514-581{mu}m$ vs. $329-472{mu}m$). A values of male are smaller (a=24.5-26.0 vs. 23.7-32.1), and the spicule length is also longer (18.2-21.0 vs. $14{mu}m$). From P. penetrans, P. gongjuensis can be distinguished by length of posterior uterine branch length having about one to four vulva body width long, but P. penetrans short and undifferentiated. P. gongjuensis differs from P. mediterraneus Corbett, 1983, which is newly recorded in Korea, in having longer posterior uterine branch ($47.6-93.8{mu}m$ vs. $18-25{mu}m$). Measurements and morphological descriptions on P. meidterraneus and P. vulnus are also provided.