RFID applied in many area. I think that RFID enlarge for our life more and more. The application technology of RFID change the Mobile RFID using mobile phone and PDF etc. I think, Mobile RFID enlarge application technology gradually. In this paper, we propose the quality measurement metrics for mobile RFID middleware. I propose the quality testing model for interoperability and security of mobile RFID middleware. We examine the international standard for mobile RFID and software testing. In this paper, I proposed the evaluation model of mobile RFID middleware on the basis of international standard ISO/IEC 9126-2 and ISO/IEC 25000 series.