This research was conducted to develop and analyze a wireless monitoring system for judging if sick or dead layers (SDL) exist in multi-tier layers battery (MLB) by machine vision, and to evaluate the performance between a wired monitoring system and it. This study used the AP (Access Point), the RS-285 to RS-232 converter, RS-232 to Ethernet converter, PICBASIC board and upgraded lump image processing method to change wired monitoring system into wireless monitoring system. The system was tested at a pilot farm and farm layer house. Results showed that monitoring judgement success rate at a pilot farm on normal cage (without SDL) was 82.3% and that on abnormal cage (with SDL) was 87.5%, respectively. And communication performance test results showed at farm layer house was $700{sim}900$ kbps while equipments operated. There were dropped slightly than performance of wired monitoring system, however, the quantity was too small to make a significant difference of performance of the controling system developed for wireless communication.