Objectives The purpose of this study is to investigate effects of Seunggal-tang gamibang (SGT) on the PMA plus A23187-induced RBL-2H3 cells. Methods The effect of SGT on ${eta}$ -hexosamonidase release, passive cutaneous anaphylaxis, $TNF-{alpha}$ production and IL-4 secretion was studied. Results SGT inhibited the release of ${eta}$ -hexosamonidase in RBL-2H3, without affecting cell viability. The passive cutaneous anaphylaxis (PCA) was inhibited by the SGT. Moreover SGT also reduced production of $TNF-{alpha}$ and secretion of IL-4 in a dose-dependent manner. Conclusions SGT has anti-allergy effects on the RBL-2H3 cells.