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Effects of Inoculum Concentration, Temperature, and Carbon Sources on Tannase Production during Solid State Fermentation of Cashew Apple Bagasse
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  • Effects of Inoculum Concentration, Temperature, and Carbon Sources on Tannase Production during Solid State Fermentation of Cashew Apple Bagasse
  • Effects of Inoculum Concentration, Temperature, and Carbon Sources on Tannase Production during Solid State Fermentation of Cashew Apple Bagasse
저자명
Rodrigues. Tigressa H.S.,Pinto. Gustavo A.S.,Goncalves. Luciana R.B.
간행물명
Biotechnology and bioprocess engineering
권/호정보
2008년|13권 5호|pp.571-576 (6 pages)
발행정보
한국생물공학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

In this study, the optimization of tannase production by solid state fermentation was investigated using cashew apple bagasse (CAB), an inexpensive residue produced by the cashew apple agroindustry, as a substrate. To accomplish this, CAB was enriched with 2.5% (w/w) tannic acid and 2.5% (w/w) ammonium sulphate and then moistened with water (60 mL/100 g of dry CAB). The influence of inoculum concentration ($10^4$ to $10^7$ spores/g), temperature (20, 25, 30, and $35^{circ}C$) and several additional carbon sources (glucose, starch, sucrose, maltose, analytical grade glycerol, and glycerol produced during biodiesel production) on enzyme production by Aspergillus oryzae was then evaluated. Supplementation with maltose and glycerol inhibited tannase synthesis, which resulted in lower enzyme activity. Starch and sucrose supplementation increased enzyme production, but decreased the enzyme productivity. The maximum tannase activity (4.63 units/g of dry substrate) was obtained at $30^{circ}C$, using $10^7$ spores/g and 1.0% (w/v) sucrose as an additional carbon source.