Habitual speaking fundamental frequency (sF0) plays an important role in determining the voice classification, which can be presented differently depending on the vocal fold length and language habits. The purpose of this study, therefore, was to compare the differences in sF0 for voice classification and closed quotient between speaking and singing. Seventeen singers (7 sopranos, 5 tenors, 5 baritones, mean age 25.1 years) with no evidence of vocal folds pathology were participated. sF0 and closed quotient (CQ) both in speaking and in singing (A3-A5 with soprano, A2-A4 with tenor and baritone) were measured using SPEAD program and electroglottography. No significant differences were observed for sF0 between tenor and baritone groups (p> 0.05). However, CQ in singing was significantly different among three groups (p< 0.05), but CQ in speaking was not (p> 0.05). Furthermore, CQ was significantly different with both soprano (p< 0.01) and tenor groups ((P= 0.02) whereas baritone group revealed there is no difference when compared between speaking and singing. No significant differences in sF0 between tenor and baritone participants may result from decision-making for voice classification by experience and should measure sF0 before determining the voice classification.