Cyanobacteria Synechocystis PCC 6803 or the extracted thylakoid membrane from this strain was appled to photosynthetic bio-electrochemical fuel cell(PBEFC) for the production of hydrogen under the illumination of 48Klux using halogen lamp. PBEFC was composed of anode, cathode and membrane between them. Electrode material was carbon paper while electron mediator and receptor were added phenazine methosulfate(PMS) and potassium ferricyanide respectively. When water and 50 mM tricine buffer and $300{mu}M$ PMS were added to the anode under the light condition, PBEFC produced the current density $4.4{ imes}10^{-5};mA/cm^2$, $1.4{ imes}10^{-4};mA/cm^2$ and $2.4{ imes}10^{-4};mA/cm^2$, respectively. And the addition of the thylakoid membrane to the system increased current density to $1.3{ imes}10^{-3};mA/cm^2$. Two times increase of the thylakoid membrane into the anode doubled the current density to $2.6{ imes}10^{-3};mA/cm^2$. But the current density was not increased proportionally to the amount of thylakoid membrane increased. The system was unstable to measure the electricity output due to the foam production in the anode. Addition of triton X-100 and tween 80 stabilized the system to measure the electricity output but the current density was not increased higher than $8.4{ imes}10^{-4};mA/cm^2$ and $2.3{ imes}10^{-3};mA/cm^2$. When the thylakoid membrane was substituted to Synechocystis PCC 6803 cells of four-day culture which has chlorophyll contents $20.5{mu}g/m{ell}$, maximum current density was $1.3{ imes}10^{-3};mA/cm^2$ with $1;k{Omega}$ resistance.