The purpose of this study is to understand the physical and chemical characteristics of biodiesel blended fuel reformed by ultrasonic energy irradiation. To do this, a mixture of commercial diesel fuel and biodiesel was compared and analyzed according to whether or not ultrasonic energy irradiation was performed and the duration of irradiation. The results of the experiments indicate that when ultrasonic energy irradiation was performed on biodiesel blended fuel, its viscosity decreased by 3-7%. In the case of BD20, when ultrasonic energy irradiation was performed, its Sauter mean diameter (SMD) dropped by 12% on average. As the irradiation duration increased, the volume ratio of olefins increased up to a maximum of 2.7%, and the higher heating value increased to a maximum of 5.8%. On the contrary, the ratio of aromatics decreased by a maximum of 2.7%, and BI decreased by a maximum of 7%.