본 연구는 유해성 Cochlodinium polykrikoides 적조생물을 대상으로 수온변화에 따른 세포 생화학적 및 생리 활성도를 측정했다. Genomic DNA 함량은 $12^{circ}C$ 및 $15^{circ}C$에서 거의 비슷한 0.6을 보였으나, $18^{circ}C$부터 급격히 높아져서 $24^{circ}C$ 최고 1.8를 나타내었다. RNA와 total protein도 $24^{circ}C$에 가장 높은 1.7과 0.07 ${mu}g$ $ml^{-1}$으로 나타났다. 광합성량도 수온에 따른 큰 변화를 보였다. 빛의 파장에 관계없이 $18^{circ}C$ 이상에서 현저히 높은 값을 보였다. $24^{circ}C$ $ETR_{max}$ Ch1-Ch4까지의 범위는 537.9에서 602.5 ${mu}mol$ electrons $g^{-1}$ Chl ${alpha}s^{-1}$ 나타났다. Nitrate reductase와 ATPase 효소 활성도는 $24^{circ}C$에서 각각 0.11 ${mu}mol$ $NO_{2}^{-}$ ${mu}g^{-1}$ Chl ${alpha}h^{-1}$ , 0.78 pmol 100 $mg^{-1}$ 나타났다. CHN 분석에서도 수온에 따라 C, H, N의 함량이 현저하게 상이했다. $27^{circ}C$ 배양시 $24^{circ}C$에 비하여 대부분의 세포생리물질이 낮게 보였다. 따라서 C. polykrikoides는 수온 변화에 대하여 세포대사물질의 함량이 많은 차이를 볼 수 있어서 초기 적조 발생 조건은 $18^{circ}C$로 추측된다. 본 실험의 결과로 $24^{circ}C$ 이상이 되면 C. polykrikoides 대번식은 세포 내 생리물질의 현저한 저하로 형성되기가 어려울 것으로 보인다.
Water temperature-dependent fluctuations of biochemical and molecular activities in the harmful dinoflagellate, Cochlodinium polykrikoides were studied. In terms of genomic DNA concentration, a similar value of 0.6 was observed at $12^{circ}C$ and $15^{circ}C$. However, DNA significantly increased beyond $18^{circ}C$ (p<0.05), to a maximum of 1.8 at $24^{circ}C$. DNA concentration significantly decreased to 0.6. The concentrations of RNA and total protein were likely at their highest values of 1.7 and 0.07 ${mu}g$ $ml^{-1}$ at $24^{circ}C$, respectively. RNA and total protein concentrations began to increase at $15^{circ}C$. Oxygen availability between lower and higher temperatures was significantly different and increased from $18^{circ}C$ according to light intensity, regardless of wavelengths (p<0.05). At $24^{circ}C$, the highest value of the maximum electron transport rate ($ETR_{max}$), ranging from 537.9 (Ch 1) to 602.5 ${mu}mol$ electrons $g^{-1}$ Chl ${alpha}s^{-1}$ (Ch 4), was also apparent. Nitrate reductase (NR) and ATPase activities were at their highest values of 0.11 ${mu}mol$ $NO_{2}^{-}$ ${mu}g^{-1}$ Chl ${alpha}h^{-1}$ and 0.78 pmol 100 $mg^{-1}$ at $24^{circ}C$, respectively. In an analysis of CHN, the concentration of C and N also significantly increased (p<0.05). Most of the measurements for the cellular activities at $27^{circ}C$, however, were less than at $24^{circ}C$. These results suggest that the sub-cellular activities of C. polykrikoides are sensitive to changes in water temperature. It may be desirable to estimate at $18^{circ}C$ the initiation of the massive blooming development of C. polykrikoides. In nature, it will be very difficult to maintain the massive blooms beyond $24^{circ}C$ because of a possibly significant decrease in molecular activity of C. polykrikoides.
